Pregled bibliografske jedinice broj: 753351
Sex-dependent expression of water channel AQP1 along the rat nephron
Sex-dependent expression of water channel AQP1 along the rat nephron // American journal of physiology. Renal physiology, 308 (2015), 8; F809-F821 doi:10.1152/ajprenal.00368.2014 (međunarodna recenzija, članak, znanstveni)
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Naslov
Sex-dependent expression of water channel AQP1 along the rat nephron
Autori
Herak-Kramberger, Carol Mirna ; Breljak, Davorka ; Ljubojević, Marija ; Matokanović, Mirela ; Lovrić, Mila ; Rogić, Dunja ; Brzica, Hrvoje ; Vrhovac, Ivana ; Karaica, Dean ; Micek, Vedran ; Ivković Dupor, Jana ; Brown, Dennis ; Sabolić, Ivan
Izvornik
American journal of physiology. Renal physiology (1931-857X) 308
(2015), 8;
F809-F821
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Western blotting; aquaporin 1; immunocytochemistry; real time RT-PCR; sex differences
Sažetak
In the mammalian kidney, non-glycosylated and glycosylated forms of water channel protein AQP1 coexist in the luminal and basolateral plasma membranes of proximal tubule and descending thin limb. Factors that influence AQP1 expression in (patho)physiological conditions are poorly known. Thus far only angiotensin II and hypertonicity were found to upregulate AQP1 expression in rat proximal tubule in vivo and in vitro (Am J Physiol Renal Physiol 297:F1575, 2009), a phenomenon that may be relevant for higher blood pressure observed in men and male experimental animals. Here we investigated the sex-dependent AQP1 protein and mRNA expression in the rat kidney by immunochemical methods and qRT-PCR in tissue samples from prepubertal and intact, gonadectomized, and sex hormone-treated gonadectomized adult male and female animals. In adult rats, the overall renal AQP1 protein and mRNA expression was ~80% and ~40% higher, respectively, in males than in females, downregulated by gonadectomy in both sexes, and upregulated strongly by testosterone- and moderately by progesterone-treatment ; estradiol- treatment had no effect. In prepubertal rats, the AQP1 protein expression was low compared to adults and slightly higher in females, whereas the AQP1 mRNA expression was low and similar in both sexes. The observed differences in AQP1 protein expression in various experiments mainly reflect changes in the glycosylated form. The male- dominant expression of renal AQP1 in rats, which develops after puberty largely in the glycosylated form of the protein, may contribute to enhanced fluid reabsorption following the androgen- or progesterone-stimulated activities of sodium- reabsorptive mechanisms in proximal tubules.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Farmacija
POVEZANOST RADA
Projekti:
022-0222148-2146 - Bubrežni prijenosnici u sisavaca; spolne razlike i učinci toksičnih metala (Sabolić, Ivan, MZOS ) ( CroRIS)
HRZZ-IP-2013-11-1481 - Starosno-ovisna ekspresija membranskih prijenosnika u štakora (AGEMETAR) (Sabolić, Ivan, HRZZ - 2013-11) ( CroRIS)
Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb
Profili:
Hrvoje Brzica
(autor)
Mirela Matokanović
(autor)
Ivana Vrhovac Madunić
(autor)
Jana Ivković
(autor)
Ivan Sabolić
(autor)
Mila Lovrić
(autor)
Davorka Breljak
(autor)
Marija Ljubojević
(autor)
Dean Karaica
(autor)
Dunja Rogić
(autor)
Carol Mirna Herak-Kramberger
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE