Naslov
Hrana obogaćena probioticima kao modulator enteralne mikrobne flore u kalifornijske pastrve (Onchorhynchus mykiss)
(Dietary probiotic supplementation as a modulator of enteral microbiota in rainbow trout Onchorhynchus mykiss))

Autori
Topić Popović, Natalija ; Strunjak-Perović, Ivančica ; Jadan, Margita ; Sauerborn Klobučar, Roberta ; Barišić, Josip ; Čož-Rakovac, Rozelinda

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / Pospišil, Milan - Zagreb : Agronomski fakultet Sveučilišta u Zagrebu, 2015, 199-200

ISBN
978-953-7878-29-0

Skup
50th Croatian and 10th International Symposium on Agriculture

Mjesto i datum
Opatija, Hrvatska, 16.02.2015. - 20.02.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
probiotici; Lactobacillus rhamnosus; kalifornijska pastrva
(probiotics; Lactobacillus rhamnosus; rainbow trout)

Sažetak
The need for sustainable aquaculture has prompted extensive research regarding the use of probiotics on aquatic organisms. Bacterium Lactobacillus rhamnosus was mixed in the trout diet with peptone water to attain two diet groups with concentrations of 10*7 CFU (G1) and 10*8 CFU (G2), respectively. The G3 feed group consisted of feed sprayed only with peptone water. First runway in line was stocked with the control group fed only the basal diet, second runway was stocked with G1, following with G2, and G3. They were fed for a period of six weeks and sampled at the beginning and end of the trial, and three weeks after the feeding trial. Before the trial, fish had no detectable lactic acid bacteria (LAB) in the intestines nor in the fecal contents. The numbers of viable lactobacilli recovered from fecal contents increased in G1 and G2 groups from below detection limits (<10 CFU/mL) at the start of the trial to levels between 2 x 10*2 and 4 x 10*5 CFU/mL (G1), between 4.2 x 10*2 and 8 x 10*2 CFU/mL (G2) by the end of the trial. Even G3 group demonstrated viable lactobacilli between neglectable to 0.6 x 10*4 CFU/mL. The feeding with supplemented probiont apparently changed the resident microbiota in fish from mostly Vibrio fluvials, Aeromonas hydrophila and Serratia fonticola before the trial, to V. fluvialis, Burkholderia cepacia, non-fermenter species, and Pasteurellaceae by the end of the trial, to V. fluvialis, Pseudomonas aeruginosa, A. hydrophila three weeks after the end of the trial.

Izvorni jezik
Hrvatski, engleski

Znanstvena područja
Veterinarska medicina, Poljoprivreda (agronomija), Biotehnologija

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