Naslov
Immune evasion potential of the murine cytomegalovirus gene m04

Autori
Lučin, Pero ; Kučić, Natalia ; Crnković, Irena ; Jonjić, Stipan ; Koszinowski, Ulrich H.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Immunology Letters (Abstract Issue) / Wagner, Hermann ; Heeg, H. ; Pfeffer, Klaus - Amsterdam : Elsevier, 1997, 354-355

Skup
13th European Immunology Meeting

Mjesto i datum
Amsterdam, Nizozemska, 22.06.1997. - 25.06.1997

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
murine cytomegalovirus; viral genes; cytomegalovirus glycoprotein; MHC class I molecules; immune evasion; NK cells

Sažetak
Introduction: Two glycoprotein families are located at the oposite ends of murine cytomegalovirus (MCMV) genome. The m02 glycoprotein family has 15 members, and at least two of them potentially interfere with antigen presentation in the MHC class I pathway. The glycoprotein of 34 kDa encoded by the m04 gene was identified by coprecipitation with MHC class i molecules from the cell surface in the IFN-gamma treated fibroblasts. Materials and Methods: Glycoprotein maruration was studied by immunoprecipitation after metabolic labelling. Cell surface expression was followed by immunoflorescence and cell surface biotinylation. Recombinant viruses were produced using cre-loxP recombination system and selected on the basis of lacZ gene exspression. Biological relevance of m04 gene was tested in vivo after infection of immunodeficient nu/nu mice and immunocompetent C57B1/6 mice. Results: gp 34 is a type 1 glycoprotein with 5 potential glycosylation sites and 3 N-linked glycand. In cells exspressing MHC class I trimolecular complexes, a proportion og the gp34 binds to MHC and is exported to the cell surface. gp34 molecules exported to the cell surface acquire only one endoH resistant N-linked glycand. If gp34 is expressed in ƒŇ-2 microglobulin deficient cells, it does not bind to MHC class I heavy chains. In these cells the gp34 does not acquire endoH resistance glycans and cannotbe exported to the cell surface without MHC class I trimolecular complexes. Experimets with MCMV-mutant in which the gp 34 is deleted have shown that m04 gene is neither responsible for the MHC class I transport block nor for backsorting of the cell surface resident MHC molecules. The immune evasion potential of the gp34 is tested in vivo by comparing virulence and organ replication of the wild type MCMV, the m04 deletion mutant and revertant virus. These studies are currently under way.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA