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Pregled bibliografske jedinice broj: 746164

HIGH THROUGHPUT ISOLATION OF IgG FROM HUMAN PLASMA SAMPLES BY USE OF CIM PROTEIN G MONOLITHIC DISKS


Pučić, Maja; Ručević, Marijana; Josić, Đuro; Lauc, Gordan
HIGH THROUGHPUT ISOLATION OF IgG FROM HUMAN PLASMA SAMPLES BY USE OF CIM PROTEIN G MONOLITHIC DISKS // MSS2010 - 4th Monolith Summer School & Symposium
Portorož, Slovenija, 2010. (poster, međunarodna recenzija, sažetak, znanstveni)


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Naslov
HIGH THROUGHPUT ISOLATION OF IgG FROM HUMAN PLASMA SAMPLES BY USE OF CIM PROTEIN G MONOLITHIC DISKS

Autori
Pučić, Maja ; Ručević, Marijana ; Josić, Đuro ; Lauc, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
MSS2010 - 4th Monolith Summer School & Symposium

Mjesto i datum
Portorož, Slovenija, 29.05.2010. - 02.06.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
protein G; IgG; monoliths

Sažetak
Immunoglobulin G is the second most abundant protein in human plasma (10-15 mg/ml). All IgG molecules carry N-glycans at the highly conserved N-glycosylation site (Asn-297) of the fragment crystalizable (Fc) part of their heavy chains. Fc N-glycosylation modulates the biological activity of IgG and furthermore changes in N-glycosylation have been found to be associated with various diseases. Using recently developed high-throughput HPLC method we want to perform a large scale study of IgG N-glycans in normal human population and use these glycans as quantitative traits for GWAS (genome wide association study) analysis to identify genes that regulate glycosylation in plasma cells. We used newly developed CIM® Protein G 96-Monolithic technology for optimization and later isolation of immunoglobulin G from 1984 human plasma samples. This affinity system consists of a unique monolithic stationary phase that is installed inside each well of an ELISA plate. Results obtained with the use of Protein G 96-Monolithic plate were comparable to those of Protein G and A bulk monolithic columns. With the proper sample preparation, optimized isolation conditions and regeneration at the end of each chromatographic run, we were able to isolate IgG from about 300 plasma samples per day. CIM® Protein G 96-Monolithic plates enable high throughput and highly efficient purification of immunoglobulin G. After this optimization step, a robotic system will be used for high throughput isolation of IgG and other less abundant proteins in order to perform further analysis of human glycoproteome.

Izvorni jezik
Engleski



POVEZANOST RADA


Ustanove:
GENOS d.o.o.


Citiraj ovu publikaciju:

Pučić, Maja; Ručević, Marijana; Josić, Đuro; Lauc, Gordan
HIGH THROUGHPUT ISOLATION OF IgG FROM HUMAN PLASMA SAMPLES BY USE OF CIM PROTEIN G MONOLITHIC DISKS // MSS2010 - 4th Monolith Summer School & Symposium
Portorož, Slovenija, 2010. (poster, međunarodna recenzija, sažetak, znanstveni)
Pučić, M., Ručević, M., Josić, Đ. & Lauc, G. (2010) HIGH THROUGHPUT ISOLATION OF IgG FROM HUMAN PLASMA SAMPLES BY USE OF CIM PROTEIN G MONOLITHIC DISKS. U: MSS2010 - 4th Monolith Summer School & Symposium.
@article{article, author = {Pu\v{c}i\'{c}, Maja and Ru\v{c}evi\'{c}, Marijana and Josi\'{c}, \DJuro and Lauc, Gordan}, year = {2010}, keywords = {protein G, IgG, monoliths}, title = {HIGH THROUGHPUT ISOLATION OF IgG FROM HUMAN PLASMA SAMPLES BY USE OF CIM PROTEIN G MONOLITHIC DISKS}, keyword = {protein G, IgG, monoliths}, publisherplace = {Portoro\v{z}, Slovenija} }
@article{article, author = {Pu\v{c}i\'{c}, Maja and Ru\v{c}evi\'{c}, Marijana and Josi\'{c}, \DJuro and Lauc, Gordan}, year = {2010}, keywords = {protein G, IgG, monoliths}, title = {HIGH THROUGHPUT ISOLATION OF IgG FROM HUMAN PLASMA SAMPLES BY USE OF CIM PROTEIN G MONOLITHIC DISKS}, keyword = {protein G, IgG, monoliths}, publisherplace = {Portoro\v{z}, Slovenija} }




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