Pregled bibliografske jedinice broj: 746153
TEMPORAL STABILITY OF INDIVIDUAL HUMAN PLASMA N-GLYCAN PROFILES
TEMPORAL STABILITY OF INDIVIDUAL HUMAN PLASMA N-GLYCAN PROFILES // The 2009 Annual Conference of the Society for Glycobiology
San Diego (CA), Sjedinjene Američke Države, 2009. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 746153 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
TEMPORAL STABILITY OF INDIVIDUAL HUMAN PLASMA N-GLYCAN PROFILES
Autori
Pučić, Maja ; Gornik, Olga ; Knežević, Ana ; Redžić, Irma ; Wagner, Jasenka ; Lauc, Gordan
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
The 2009 Annual Conference of the Society for Glycobiology
Mjesto i datum
San Diego (CA), Sjedinjene Američke Države, 12.11.2009. - 15.11.2009
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
glycans; stability
Sažetak
Almost all plasma proteins are glycosylated and their glycan parts can exist in various structural forms, resulting in different glycoforms of the same molecule. Glycan heterogeneity was shown to be connected to many diseases and glycosylation holds an unbelievable diagnostic potential due to main biological features and structural characteristics of glycans. Recently we observed high biological variability of human plasma N-glycome at population level. Thus, it was of great importance to standardize analytical methods and examine the temporal constancy of N-glycome in human individuals before routine implementation of techniques that determine glycosylation changes in diagnostic laboratories. Plasma samples were taken from 12 healthy individuals. The blood was drawn on seven occasions during five days. N-linked glycans, released from plasma proteins, were separated using hydrophilic interaction high performance liquid chromatography into 16 groups (GP1-GP16) and quantified. The results showed very small variation in all glycan groups, indicating very good temporal stability of Nglycome in individual. Coefficients of variation from 1.6% for GP8 to 11.4% for GP1, with average of 5.6%, were obtained. These variations were comparable to those due to limitations in the experimental methods when analytical procedure was tested for its precision. We conclude that plasma N-glycome in each individual has very good stability which implies that it is under genetic regulation. Changes occurring in glycan profiles are consequence of environmental influences and physiologic responses and therefore carry good potential diagnostic value.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
GENOS d.o.o.,
Sveučilište u Zagrebu
Profili:
Maja Pučić Baković
(autor)
Olga Gornik Kljaić
(autor)
Gordan Lauc
(autor)
Jasenka Wagner
(autor)
Ana Mužinić
(autor)