Naslov
STABILITY OF N-GLYCAN PROFILES IN HUMAN PLASMA

Autori
Gornik, Olga ; Knežević, Ana ; Pučić, Maja ; Redžić, Irma ; Wagner, Jasenka ; Lauc, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
EMBO Young Scientists Forum

Mjesto i datum
Zagreb, Hrvatska, 15.06.2009. - 17.06.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
glycans; stability

Sažetak
Almost all plasma proteins are glycosylated and their glycan parts can exist in various structural forms, resulting in different glycoforms of the same molecule. Glycan heterogeneity was shown to be connected to many diseases and glycosylation holds an unbelievable diagnostic potential due to main biological features and structural characteristics of glycans. Recently we observed high biological variability of human plasma N-glycome at population level. Thus, it was of great importance to standardize analytical methods and examine the temporal constancy of N-glycome in human individuals before routine implementation of techniques that determine glycosylation changes in diagnostic laboratories. Plasma samples were taken from 12 healthy individuals. The blood was drawn on seven occasions during five days. N-linked glycans, released from plasma proteins, were separated using hydrophilic interaction high performance liquid chromatography into 16 groups (GP1-GP16) and quantified. The results showed very small variation in all glycan groups, indicating very good temporal stability of Nglycome in individual. Coefficients of variation from 1.6% for GP8 to 11.4% for GP1, with average of 5.6%, were obtained. These variations were comparable to those due to limitations in the experimental methods when analytical procedure was tested for its precision. We conclude that plasma N-glycome in each individual has very good stability which implies that it is under genetic regulation. Changes occurring in glycan profiles are consequence of environmental influences and physiologic responses and therefore carry good potential diagnostic value.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA