Pregled bibliografske jedinice broj: 746136
STABILITY OF N-GLYCAN PROFILES IN HUMAN PLASMA
STABILITY OF N-GLYCAN PROFILES IN HUMAN PLASMA // The Sixth ISABS Conference on human genome project based applications in Forensic Science, Anthropology and Individualized medicine
Split, Hrvatska, 2009. (poster, međunarodna recenzija, sažetak, znanstveni)
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Naslov
STABILITY OF N-GLYCAN PROFILES IN HUMAN PLASMA
Autori
Gornik, Olga ; Knežević, Ana ; Pučić, Maja ; Redžić, Irma ; Wagner, Jasenka ; Lauc, Gordan
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
The Sixth ISABS Conference on human genome project based applications in Forensic Science, Anthropology and Individualized medicine
Mjesto i datum
Split, Hrvatska, 01.06.2009. - 05.06.2009
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
glycans; stability
Sažetak
Glycan heterogeneity was shown to be associated with numerous diseases and glycan analysis has a great diagnostic potential. Recently, we reported high biological variability of human plasma N-glycome at the level of population. The observed variations were larger than changes reported to be associated with some diseases ; thus, it was of great importance to examine the temporal constancy of human N-glycome before glycosylation changes could be routinely analyzed in diagnostic laboratories. Plasma samples were taken from 12 healthy individuals. The blood was drawn on seven occasions during 5 days. N-Linked glycans, released from plasma proteins, were separated using hydrophilic interaction high performance liquid chromatography into 16 groups (GP1-GP16) and quantified. The results showed very small variation in all glycan groups, indicating very good temporal stability of N-glycome in a single individual. Coefficients of variation from 1.6% for GP8 to 11.4% for GP1 were observed. The average coefficient of variation was 5.6%. These variations were comparable to those observed when analytical procedure was tested for its precision. Good stability of plasma N-glycome in healthy individuals implies that glycosylation is under significant genetic control. Changes observed in glycan profiles are consequence of environmental influences and physiologic responses and therefore have a significant diagnostic potential.
Izvorni jezik
Engleski
POVEZANOST RADA
Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
GENOS d.o.o.
Profili:
Maja Pučić Baković
(autor)
Olga Gornik Kljaić
(autor)
Jasenka Wagner
(autor)
Gordan Lauc
(autor)
Ana Mužinić
(autor)