Naslov
EXTRACELLULAR ENRICHMENT OF SPECIFIC NONVIRION MCMV PROTEINS

Autori
Grabušić, Kristina ; Juranić Lisnić, Vanda ; Popović, Branka ; Kučić, Natalia ; Brizić, Ilija ; Jonjić, Stipan ; Lučin, Pero

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2014 Annual Meeting of the Croatian Immunological Society (Book of abstracts) / Boja Polić i sur. - Rijeka, 2014, 52-52

Skup
Godišnji skup Hrvatskog imunološkog društva (Annual Meeting of the Croatian Immunological Society)

Mjesto i datum
Krk, Hrvatska, 17.10.2014. - 18.10.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
microvesicles; exosomes; cytomegalovirus

Sažetak
Cells have the ability to secrete various membrane vesicles into extracellular environment either by shedding them from plasma membrane or by releasing endosome-derived microvesicles from the intracellular multivesicular body.The secreted microvesicles can deliver proteins and/or nucleic acids to other cells and influence diverse biologic processes including cell proliferation, apoptosis, gene expression and immune regulation. Viral infections can change composition of secreted microvesicles by targeting macromolecules of both cellular and viral origin for secretion. Depending on the viral infection extracellular microvesicles can act either in favour or against maintenance as well spread of viral infection. Microvesicles secreted from infected cells were already described for human cytomegalovirus (CMV), but not for mouse CMV (MCMV). Here we show that MCMV infection of primary mouse embryonic fibroblasts results in enrichment of cellular CD63 protein in the conditioned cell medium. This transmembrane protein is marker of a specific class of extracellular microvesicles named exosomes. In addition, the isolated fraction of conditioned medium contained several nonvirion viral proteins including m04, m06 and IE, but not m152 nor E1. None of the tested proteins associated with MCMV virions, including m57, m55 and m45, were present in the isolated fraction of the conditioned medium.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA