Naslov
Transcriptional analysis of the recA gene in Streptomyces rimosus: identification of a new type of promoter

Autori
Ahel, Ivan ; Vujaklija, Dušica ; Mikoč, Andreja ; Gamulin, Vera

Izvornik
FEMS microbiology letters (0378-1097) 209 (2002), 1; 129-133

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
recA gene ; Promoter ; Transcription ; UV-induction ; Streptomyces rimosus

Sažetak
Using primer-extension analysis we identified two transcription start sites for the recA gene in Streptomyces rimosus. A longer, weak transcript is initiated from the distal SEP promoter that contains a Cheo box like sequence: GAAC-N4-ATTC. However, the major start site of transcription is a G at position −36 and this shorter transcript significantly increases in response to DNA damage by UV-light. The −35 box (TTGTCA) and −10 box (TAGCGT) of the strong recA promoter are only 11 bp apart and this proximal promoter is almost identical to the strong, DNA damage-inducible promoter of Mycobacterium tuberculosis recA gene. We inspected the Streptomyces coelicolor database and found this type of promoter in the upstream regions of many (potentially) UV-inducible genes as well as some other genes/ORFs. Moreover, the DNA sequence between the predicted −35 and −10 boxes is also partially conserved. The consensus sequence for this new type of promoter in Streptomyces is: TTGTCAGTGGC-N6-TAGggT.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA