ࡱ> '1&#` **bjbjmm u;_   8 ,6L * "" ")))))))$,h /*9 "Z"Z"@""* =*r'r'r'"P<)r'")r'r' |r' x%r'2)S*0*r'/%6/r'r't/ 'L""r'"""""**'^"""*""""  $   Godianji skup Hrvatskog imunoloakog druatva, Krk 2014. Obrazac za prijavu sa~etaka Naslov teme:CELL BIOLOGY OF MCMV-INFECTED MICROGLIA  the model establishment Autor(i): (podvui izlagatelja)Natalia Ku i1, Kristina Grabuai2, Vlatka Sotoaek Tokmad~i3 , Vedrana Montana Parpura2 Ustanova:1Department of Physiology and Immunology, Faculty of Medicine, University of Rijeka, Brae Branchetta 20, Rijeka, Croatia 2Department of Biotechnology, University of Rijeka, Radmile Matej i 2, Rijeka, Croatia 3Department of Anesthesiology, Resustitution and Intensive Care Medicine, Faculty of Medicine, University of Rijeka, Brae Branchetta 20, Rijeka, CroatiaOralna prezentacija DA/NENO The central nervous system (CNS) is one of the principal target organs for cytomegalovirus (CMV) infection during neurodevelopment and immunosuppression. Current concept regarding the pathogenesis of neurological injury caused by CMV includes glial cell-mediated defenses. Among glia, microglia cells, the resident immune cells in the brain, are sensors of viral infection. They initiate a cascade of neuroimmune responses that result in defense of damaged brain. In response to CNS infections, microglial cells can quickly transform from a quiescent (ramified) state into an activated (amoeboid) state, acquiring macrophage phenotype and critical effector functions required to launch effective immune responses. Our investigations recently turned from studies on antigen presenting properties of murine CMV (MCMV)-infected primary mouse embryonic fibroblasts (MEFs) to MCMV-infected murine microglia cells model. For the purposes of these experiments, we established cell culture system model which implies MCMV-infected murine microglia BV-2 cell line. This microglial cell line is widely used whereas analysis on characterisation revealed a high similarity to primary microglial cells. Since BV-2 cells are easy to culture, they are a valuable tool to study inflammatory processes and neuroimmune response. Results from our recent study with MCMV show that BV-2 cells are fully permissive to MCMV replication but expression of early viral immunoevasion gene products (m152, m04, m06) is relatively postponed to well establish model on MEFs. 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