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Pregled bibliografske jedinice broj: 739291

Modification of comet-FISH technique by using temperature instead of chemical denaturation

Mladinić, Marin; Želježić, Davor
Modification of comet-FISH technique by using temperature instead of chemical denaturation // MethodsX (Amsterdam), 1 (2014), 162-167 doi:10.1016/j.mex.2014.080.010 (podatak o recenziji nije dostupan, članak, ostalo)

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Naslov
Modification of comet-FISH technique by using temperature instead of chemical denaturation

Autori
Mladinić, Marin ; Želježić, Davor

Izvornik
MethodsX (Amsterdam) (2215-0161) 1 (2014); 162-167

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, ostalo

Ključne riječi
Comet-FISH; TP53; Temperature denaturation; Lymphocytes; Primary DNA damage; Gene fragmentation

Sažetak
Comet-FISH technique is an extension of commonly used comet assay.Its purpose is to determine whether primary DNA damage which comet assay detects occurred within a sequence of interest that is visualized by hybridization of fluorescent probe. Presence of the signal in comet tail indicates impaired structural integrity of sequence. Our modifications to the original comet-FISH technique described by Rappet al.(2000) [1] include:  increase in probe binding specificity,  increased rate of successful hybridization,  simultaneous temperature denaturation of both, slide and probe.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA

Projekti:
022-0222148-2137 - Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla (Kašuba, Vilena, MZOS ) ( CroRIS)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb

Profili:

Avatar Url Davor Želježić (autor)

Avatar Url Marin Mladinić (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Mladinić, Marin; Želježić, Davor
Modification of comet-FISH technique by using temperature instead of chemical denaturation // MethodsX (Amsterdam), 1 (2014), 162-167 doi:10.1016/j.mex.2014.080.010 (podatak o recenziji nije dostupan, članak, ostalo)
Mladinić, M. & Želježić, D. (2014) Modification of comet-FISH technique by using temperature instead of chemical denaturation. MethodsX (Amsterdam), 1, 162-167 doi:10.1016/j.mex.2014.080.010.
@article{article, author = {Mladini\'{c}, Marin and \v{Z}elje\v{z}i\'{c}, Davor}, year = {2014}, pages = {162-167}, DOI = {10.1016/j.mex.2014.080.010}, keywords = {Comet-FISH, TP53, Temperature denaturation, Lymphocytes, Primary DNA damage, Gene fragmentation}, journal = {MethodsX (Amsterdam)}, doi = {10.1016/j.mex.2014.080.010}, volume = {1}, issn = {2215-0161}, title = {Modification of comet-FISH technique by using temperature instead of chemical denaturation}, keyword = {Comet-FISH, TP53, Temperature denaturation, Lymphocytes, Primary DNA damage, Gene fragmentation} }
@article{article, author = {Mladini\'{c}, Marin and \v{Z}elje\v{z}i\'{c}, Davor}, year = {2014}, pages = {162-167}, DOI = {10.1016/j.mex.2014.080.010}, keywords = {Comet-FISH, TP53, Temperature denaturation, Lymphocytes, Primary DNA damage, Gene fragmentation}, journal = {MethodsX (Amsterdam)}, doi = {10.1016/j.mex.2014.080.010}, volume = {1}, issn = {2215-0161}, title = {Modification of comet-FISH technique by using temperature instead of chemical denaturation}, keyword = {Comet-FISH, TP53, Temperature denaturation, Lymphocytes, Primary DNA damage, Gene fragmentation} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Emerging Sources Citation Index (ESCI)
  • Scopus

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