Naslov
The effect of p73 isoforms on DNA methylation in cancer cells

Autori
Tučkar, Neven ; Zorić, Arijana ; Medvedović, Mario ; Butorac, Dražan ; Eljuga, Damir ; Slade, Neda

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Periodicum Biologorum, HDIR-3 Third Meeting with International Participation “From Bench to Clinic” / Ozretić, Petar ; Levanat, Sonja - Zagreb : Hrvatsko prirodoslovno društvo, 2014, 58-58

Skup
HDIR-3 Third Meeting with International Participation “From Bench to Clinic”

Mjesto i datum
Zagreb, Hrvatska, 06.11.2014. - 07.11.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
p73 isoforms; global DNA methylation

Sažetak
The role of p73 in tumorigenesis is not fully understood. Using P1 promoter transactivating (TAp73) isoforms are generated, and P2 promoter the isosforms without entire transactivation domain (ΔNp73). The most common change in tumors is increased expression of ΔNp73, which acts as transdominant inhibitor of TAp73 and p53. DNA methylation is a form of epigenetic regulation, which adds methyl groups to cytosine residues, regulating gene activity. Hypermethylation of CpG islands in promoter regions inhibits transcription of genes silencing its expression. Using inducible tet-on system, we examined the impact of increased expression of TAp73α and ΔNp73α on global DNA methylation using Illumina Human Methylation 450 BeadChip. Statistically significant change was not found upon induced expression of any p73 isoform.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA