Naslov
Sensing the shape of the ds-DNA/RNA structures by non-covalent interactions

Autori
Piantanida, Ivo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts: 7th Central European Conference "Chemistry towards Biology” / Prof. Jaroslaw Polanski - Katovice, 2014, 5-5

Skup
7th Central European Conference "Chemistry towards Biology”

Mjesto i datum
Katowice, Poljska, 09.09.2014. - 13.09.2014

Vrsta sudjelovanja
Plenarno

Vrsta recenzije
Nije recenziran

Ključne riječi
DNA ; RNA ; recognition ; supramolecular chemistry

Sažetak
Small molecules targeting DNA and RNA have attracted significant scientific interest not only because of their biomedicinal applications, but also due to widespread use of spectrophotometric markers in the related scientific research – for instance, fluorescent techniques significantly developed during the last two decades and now represent about 60% of the detection enabling technologies used in molecular biology and medicine. Small molecules targeting double stranded (ds)-DNA/RNA often rely on the one dominant non-covalent binding mode for their interaction (e.g., intercalation, minor or major groove binding, external electrostatic binding). However, the combinations of different binding modes as well as additional steric and structural features controlling three-dimensional recognition were intensively explored within the last decades. Our research was particularly focused on the small molecules binding via non-covalent interactions (logKs > 5 M-1) to the most of naturally occurring ds-DNA and ds-RNA secondary structures and yielding different signals for each of the most common secondary structures (e.g. A-DNA/RNA, B-DNA, subtypes of DNA characterized by narrower minor groove, etc.) by sensitive and biologically applicable methods (UV/Vis, CD, fluorescence). The development of such single-molecule sensors, able to report simultaneously the presence of several different secondary structures of ds-DNA and ds-RNA species by different spectroscopic signals could replace the necessary application of several dyes, each one specific for one ds-DNA or ds-RNA target. Obtained results point out that the such multipurpose signalling selectivity can be achieved by fine tuning of small molecule structure (steric and DNA/RNA-binding/repulsion contacts) in respect to the slightly different binding sites of various ds-polynucleotides.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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