Naslov
SPE method for separation of lipid classes: comparative study

Autori
Milošević, Ana ; Giacometti, Jasminka ; Milin, Čedomila

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HTC 7, Seventh International Symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers / - Briž : Royal Flemish Chemical Society, 2002

Skup
HTC 7, Seventh International Symposium on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers

Mjesto i datum
Brugge, Belgija, 06.02.2002. - 08.02.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
SPE sorbents; lipids; polar lipids; polar lipid fatty acids; regiospecific TAG analysis

Sažetak
A solid phase extraction method (SPE) was developed and validated to enable the separation of lipid mixture into individual classes in high yield and purity. Three solid sorbents, based on styrene divinylbenzene/methacrylate copolymer (Nexus), octadecylsilica (C18) and aminopropylsilica (NH2) were compared as effective tools for the SPE separation of lipid classes: polar lipids (PL), sterols, monoacylglycerols (MAG), diacylglycerols (DAG) and triacylglycerols (TAG). The recoveries of lipids on Nexus were very low and this sorbent can not be used in future analysis of above-mentioned compounds. The obtained results suggest that aminopropylsilica SPE columns, used with appropriate solvent regime, are more effective as a sorbent and extracting tool for the separation of examined lipids into the classes and that the proposed method can be used as micro-procedure for a quick lipid preparation in analysis of edible oils, biological tissue samples and in regiospecific analysis of TAG by 1, 3-specific lipase hydrolysis.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA