Naslov
Alginate a stabile and biocompatible stem cells-seeded hydrogel carrier for the targeted delivery in the stroke lesion

Autori
Ferhatović Hamzić, Lejla ; Lovrić, Marija ; Casarosa, Simona ; Gajović Srećko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Bridges in Life Sciences 9th Annual Scientific Conference / - : RECOOP HST Association, 2014, 54-54

ISBN
978-963-08-9491-3

Skup
RECOOP: Bridges in Life Sciences 9th Annual Scientific Conference

Mjesto i datum
Split, Hrvatska, 27.05.2014. - 01.06.2014

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
neural stem cells; alginate; stroke; mouse

Sažetak
Stroke-induced lesion site is characterized by necrotic loose tissue which can accept relatively high volume injections without damaging brain tissue, and thus it is appealing site for therapeutic actions. Stem cell therapy for tissue regeneration and replacement of the lost cells after stroke can be directed to lesion site by using different hydrogels as a physical and biological support for cells. Natural polysaccharide alginate was proved to improve biological fate of cells in the three dimensional cultures. We hypothesised that encapsulation of neural stem cells (NSCs) within alginate matrix will enhance their viability, proliferation, differentiation in vitro and provide a stabile physical support for the cells in vivo. NSCs were isolated from the brains of 14 days old mouse fetuses and cell encapsulation was achieved by mixing the cell slurry with alginate and dropping into a 0.1M calcium chloride solution for 10 min. Soft alginate bead size was standardized to 3 mm in diameter. After 9 days following encapsulation, immunocytochemistry, live/dead test and DNA quantification tests were performed. Encapsulated NSCs were stained with PKH26 stain and then injected stereotaxically to the mouse brain. After the injection on day 1 and day 3 Nissl staining and light and fluorescence microscopy were performed. NSCs were viable 9 days after encapsulation within alginate beads and the number of cells increased by five folds. They also expressed Map 2 marker, confirming the differentiation to neurons. PKH26 stain and Nissl staining showed alginate hydrogel- seeded NSC population at the injection site 1 and 3 days after the injection. Our results proved efficacy of alginate hydrogel in improving NSCs biological outcome in vitro. Moreover, it also provides a stabile physiological structure to entrap NSCs at the injection site, where their therapeutic activity is needed. Alginate hydrogel may serve as stabile and biocompatible physical support of NSCs in the lesion site-directed stem cell therapy after stroke.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA