Pregled bibliografske jedinice broj: 736830
Mn porphyrin-based SOD mimic, MnTnHex-2-Pyp(5+) and non-SOD mimic MnTBAP(3-) suppressed rat spinal cord ischemia/reperfusion injury via NF-kB pathways
Mn porphyrin-based SOD mimic, MnTnHex-2-Pyp(5+) and non-SOD mimic MnTBAP(3-) suppressed rat spinal cord ischemia/reperfusion injury via NF-kB pathways // Free radical research, 48 (2014), 12; 1426-1442 doi:10.3109/10715762.2014.960865 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 736830 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Mn porphyrin-based SOD mimic, MnTnHex-2-Pyp(5+) and non-SOD mimic MnTBAP(3-) suppressed rat spinal cord ischemia/reperfusion injury via NF-kB pathways
Autori
Ćelić, Tanja ; Španjol, Josip ; Bobinac, Mirna ; Tovmasyan, Artak ; Vukelić, Iva ; Reboucas, Julius ; Batinić-Haberle, Ines ; Bobinac, Dragica
Izvornik
Free radical research (1071-5762) 48
(2014), 12;
1426-1442
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Mn porphyrin; spinal cord injury; ischemia/reperfusion
Sažetak
Herein we have demonstrated that both superoxide dismutase (SOD) mimic, cationic Mn(III) meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin (MnTnHex-2-PyP5+), and non-SOD mimic, anionic Mn(III) meso-tetrakis(4-carboxylatophenyl)porphyrin (MnTBAP3−), protect against oxidative stress caused by spinal cord ischemia/reperfusion via suppression of nuclear factor kappa B (NF-κB) pro-inflammatory pathways. Earlier reports showed that Mn(III) N-alkylpyridylporphyrins were able to prevent the DNA binding of NF-κB in an aqueous system, whereas MnTBAP3− was not. Here, for the first time, in a complex in vivo system—animal model of spinal cord injury—a similar impact of MnTBAP3−, at a dose identical to that of MnTnHex-2-PyP5+, was demonstrated in NF-κB downregulation. Rats were treated subcutaneously at 1.5 mg/kg starting at 30 min before ischemia/reperfusion, and then every 12 h afterward for either 48 h or 7 days. The anti-inflammatory effects of both Mn porphyrins (MnPs) were demonstrated in the spinal cord tissue at both 48 h and 7 days. The downregulation of NF-κB, a major pro-inflammatory signaling protein regulating astrocyte activation, was detected and found to correlate well with the suppression of astrogliosis (as glial fibrillary acidic protein) by both MnPs. The markers of oxidative stress, lipid peroxidation and protein carbonyl formation, were significantly reduced by MnPs. The favorable impact of both MnPs on motor neurons (Tarlov score and inclined plane test) was assessed. No major changes in glutathione peroxidase- and SOD-like activities were demonstrated, which implies that none of the MnPs acted as SOD mimic. Increasing amount of data on the reactivity of MnTBAP3− with reactive nitrogen species (RNS) (.NO/HNO/ONOO−) suggests that RNS/MnTBAP3−-driven modification of NF-κB protein cysteines may be involved in its therapeutic effects. This differs from the therapeutic efficacy of MnTnHex-2-PyP5+ which presumably occurs via reactive oxygen species and relates to NF-κB thiol oxidation ; the role of RNS cannot be excluded.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
062-0620226-0207 - Promjene koštanog metabolizma u bolestima bubrega i jetre (Bobinac, Dragica, MZOS ) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka
Profili:
Ines Batinić-Haberle
(autor)
Josip Španjol
(autor)
Dragica Bobinac
(autor)
Tanja Ćelić
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE