Naslov
The presence of virulence factors in quinolone resistant uropatogenic Escherichia coli in outpatients with UTI

Autori
Barišić, Zvonimir ; Plečko, Vanda ; Budimir, Ana ; Bedenić, Branka ; Bošnjak, Zrinka ; Kaliterna, Vanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Clin Microbiol Infect 2012 ; 18 (suppl. 3) / - , 2012, 487-487

Skup
22th European Congress of Clinical Microbiology and Infection

Mjesto i datum
London, Ujedinjeno Kraljevstvo, 31.03.2012. - 03.04.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Escherichia coli; quinolone; UTI

Sažetak
Objectives: Urinary tract infections are among the most common human infections and Escherichia coli is their main cause. Uncontrolled use of quinolones leads to resistance to this group of antibiotic. Aim of this study was to get an insight of characteristics of quinolone-resistant E. coli, taking into consideration presence of virulence factors (hemolysin, P fimbriae, type 1 fimbriae, cytotoxic necrotizing factor 1), as well as to determine difference in characteristics of strains that are quinolone-susceptible. Methods: During one year period all quinolone-resistant E. coli strains (n=87) were collected from urine samples obtained from outpatients with symptoms of urinary tract infections in the Split and Dalmatia County, Croatia. Control group (n=87) was formed by taking the next isolated quinolone-susceptible strain of E. coli for each quinolone-resistant strain. Virulence factors are determined by the molecular or phenotypic methods. Production of hemolysin was determined by phenotypic method, by observing the presence of hemolysis near the colony of the tested strain on blood agar. The presence of P fimbriae, type 1 fimbriae, and cytotoxic necrotizing factor 1 was proven by polymerase chain reaction, determining the presence of genes pap, fim and cnf1. The statistical significance of the difference between quinolone-resistant E. coli strains and quinolone-susceptible strain of E. coli was determined by Chi-square test Results: Virulence factors were found statistically significantly less frequently (all P <0.002) in quinolone-resistant strains (fim gene in 21.84%, pap gene in 20.69%, cnf1 gene in 1.15% and hemolysis in 2.30% strains) than in quinolone-sensitive strains (fim gene in 90, 80%, pap gene in 50.57%, cnf1 gene in 12.64% and hemolysis in 52.87% strains). Conclusions: There are numerous clinical observations that quinolone - resistant strains of E. coli are less virulent and more frequently cause cystitis and asymptomatic bacteriuria than invasive urinary tract infections like pyelonephritis and febrile urinary tract infections. Studies in the available literature that differentiate the presence of virulence factors in strains resistant to quinolones and their presence in sensitive strains are rare. Results of this study confirm that the virulence factors are significantly less present in quinolone - resistant strains than in quinolone - sensitive strains.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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