Naslov
Application of alcaline single cell gel electrophoresis (SCGE) assay in assessment of DNA damage in peripheral blood leukocytes of radar-facility workers

Autori
Garaj-Vrhovac, Vera ; Kopjar, Nevenka ; Želježić, Davor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Genetic Sscepribility at low Doe Eposure; Abstracts / - Ghent, 2001

Skup
31st Annual Meeting of European Environmental Mutagen Society

Mjesto i datum
Gent, Belgija, 01.09.2001. - 05.09.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Sažetak
The lakaline single cell gel electrophoresis (SCGE) assay was employed to assess the levels of DNA damage in peripheral blood leukocytes of radar-facility workers daily exposed to microwave radiation and unexposed control subjects. To quantify the DNA damage the comet tail length and the tail moment were evaluated. In peripheral blood leukocytes of the exposed subjects the alkaline SCGE assay showed larger amount of DNA migration, expressed by tail length and tail moment. Between the levels of DNA damage recorded in exposed subjects individual variations were also observed. Our results suggest that long time occupational exposure to microwave radiation could be able of causing genome damage in somatic cells and therefor it may represent a potential hazard to human health. The results obtained confirm usefulness of the alkaline SCGE assay as a sensitive biomarker of exposure. Together with different cytogentic techniques it provides a powerful technique for rapid detection of primary DNA lesions in peripheral blood leukocytes of population occupationally exposed to microwave radiation.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

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