Naslov
Cytogenetic biomonitoring of a Croatian population occupationally exposed to complex mixture of pesticides

Autori
Garaj-Vrhovac, Vera ; Želježić, Davor

Izvornik
Toxicology 165 (2001), 2-3; 153-162

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
chromosomal aberrations; sister chromatid exchanges; micronucleus frequency; comet assay; pesticides; biomonitoring

Sažetak
This paper describes a longitudinal study of possible genetic damage in Croatian workers occupationally exposed to a complex mixture of pesticides. The methods of choice were chromosomal aberration analysis, sister chromatid exchange analysis (SCE), micronucleus assay and comet assay. In order to determine primary genotoxic effects in workers, blood samples were taken after the workers spent 8 months in production of pesticides. During the production all subjects were simultaneously exposed to a complex mixture of pesticides containing atrazine, alachlor, 2,4-dichlorophenoxyacetic acid, and malathion. To detect DNA repair in lymphocytes of the same subjects the second series of blood samples was taken 8 months after the workers were removed from production. Regardless of the sampling time, the exposed workers showed an increased number of chromosomal aberrations, SCE frequency, micronucleus frequency, and values of comet assay parameters. After 8 months of non-exposure the workers showed a significantly decreased number of chromosomal aberrations, MN frequency, and DNA migration compared to the results of the first sampling, but it was still significantly higher than in controls. Furthermore, the SCE frequency in the exposed subjects did not drop after 8 months of non-expossure, which indicates long-term exposure to a mixture of pesticides.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

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