Naslov
Bioactivity of Satureja montana L. honey extracts and their profile screening

Autori
Malenica Staver, Mladenka ; Ratkaj, Ivana ; Broznić, Dalibor ; Jerković, Igor ; Marijanović, Zvonimir ; Željezić, Davor ; Kraljević Pavelić, Sandra

Izvornik
RSC Advances (2046-2069) 4 (2014); 47329-47340

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Satureja montana honey; apoptosis; cell cycle; antiproliferative; tumour
(Vrijesak med; Satureja montana; apoptoza; stanični ciklus; antiproliferativni učinak; tumor)

Sažetak
Exploitation of natural compounds, including those from the honeys, as therapeutic agents is still gaining importance, but problems may arise due to poor knowledge of molecular effects and correlation of these effects with doses. The aim of the study was to characterize the extracts from winter savory (Satureja montana L.) honey, evaluate their bioactivity depending on the variety of obtained extracts, concentrations of main compounds and their interrelationship depending on doses. For this purpose the honey samples were processed by ultrasonic solvent extraction (solvent pentane : diethyl ether 1 : 2 v/v) and the obtained extracts were characterized by gas chromatography and mass spectrometry. For the first time, antioxidative and antiproliferative effects accompanied by apoptosis induction and cell death of S. montana honey samples and their extracts on tumour cell lines were presented. Methyl syringate (MS) and syringaldehyde (SA) were identified as the major components of S. montana honey extracts with radical scavenging capacity (Trolox equivalent antioxidant capacity ; TEAC) of 13.04 mmol TEAC per kg (MS) and 422.02 mmol TEAC per kg (SA), respectively. Tested extracts, MS and SA did not exhibit any genotoxic effects detectable by the alkaline comet assay but they, as well as S. montana honey samples, clearly pointed to a strong antiproliferative effect in vitro on the tumour cell lines growth in a concentration-dependent manner accompanied with apoptosis induction and cell death. Introduction Biologically active natural compounds have been studied for decades due to their positive effects on human health and chemopreventive properties. Among 175 small molecules for tumour treatment, 74.8% are other than synthetic and 48.6% are either natural products or directly derived from them.1 Honey has been historically perceived as a natural promoter of human health. It has been used for centuries as a food source of high nutritional value and for various therapeutic purposes.2 In particular, it reduces cardiovascular risk factors and exhibits antimicrobial, anti-inammatory, antioxidant, cytostatic, antimutagenic, antitumour, immunosuppressive and growth stimulating properties.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

Napomena
Correction - DOI: 10.1039/C4RA90043J

POVEZANOST RADA