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Pregled bibliografske jedinice broj: 725573

Archaeal aminoacyl-tRNA synthetases interact with the ribosome to recycle tRNAs


Godinić Mikulčić, Vlatka; Jarić, Jelena; Greber, J., Basil; Franke, Vedran; Hodnik, Vesna; Anderluh, Gregor; Ban, Nenad; Weygand- Đurašević, Ivana
Archaeal aminoacyl-tRNA synthetases interact with the ribosome to recycle tRNAs // Nucleic acids research, 42 (2014), 8; 5191-5201 doi:10.1093/nar/gku164 (međunarodna recenzija, članak, znanstveni)


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Naslov
Archaeal aminoacyl-tRNA synthetases interact with the ribosome to recycle tRNAs

Autori
Godinić Mikulčić, Vlatka ; Jarić, Jelena ; Greber, J., Basil ; Franke, Vedran ; Hodnik, Vesna ; Anderluh, Gregor ; Ban, Nenad ; Weygand- Đurašević, Ivana

Izvornik
Nucleic acids research (0305-1048) 42 (2014), 8; 5191-5201

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
aminoacyl-tRNA synthetase ; protein complex ; ribosome ; tRNA ; SPR ; thermophoresis

Sažetak
Aminoacyl-tRNA synthetases (aaRS) are essential enzymes catalyzing the formation of aminoacyl- tRNAs (aa-tRNAs), the immediate precursors for encoded peptides in ribosomal protein synthesis. Previous studies have suggested a link between tRNA aminoacylation and high- molecular-weight cellular complexes such as the cytoskeleton or ribosomes. However, the structural basis of these interactions and potential mechanistic implications are not well understood. To biochemically characterize these interactions we have used a system of two interacting archaeal aaRSs ; an atypical methanogenic type SerRS (mSerRS) and an archaeal ArgRS. We have shown that these two aaRSs bind to the large ribosomal subunit with micromolar affinities. More specifically, we identify the L7/L12 stalk and the proteins located near the stalk base as the main sites for aaRS binding. Finally, we have performed a bioinformatics analysis of synonymous codons in the Methanothermobacter thermautotrophicus genome that supports a mechanism in which the deacylated tRNAs may be recharged by aaRSs bound to the ribosome and reused at the next occurrence of a codon encoding the same amino acid. These results suggest a mechanism of tRNA recycling in which aaRSs associate with the L7/L12 stalk region of the ribosome to recapture the tRNAs released from the preceeding ribosome in polysomes.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekti:
FP7 Regpot/IntegraLife
no. 315997
bilateral collaboration programme between Slovenia and Croatia (ARRS BI-HR/12-13-025
119-0982913-1358 - Strukturna raznolikost seril-tRNA sintetaza i točnost biosinteze proteina (Rokov Plavec, Jasmina; Weygand Đurašević, Ivana, MZOS ) ( CroRIS)
HRZZ-IS-09.01/293 - Nekanonske uloge aminoacil-tRNA-sintetaza

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

doi nar.oxfordjournals.org www.ncbi.nlm.nih.gov

Citiraj ovu publikaciju:

Godinić Mikulčić, Vlatka; Jarić, Jelena; Greber, J., Basil; Franke, Vedran; Hodnik, Vesna; Anderluh, Gregor; Ban, Nenad; Weygand- Đurašević, Ivana
Archaeal aminoacyl-tRNA synthetases interact with the ribosome to recycle tRNAs // Nucleic acids research, 42 (2014), 8; 5191-5201 doi:10.1093/nar/gku164 (međunarodna recenzija, članak, znanstveni)
Godinić Mikulčić, V., Jarić, J., Greber, J., Basil, Franke, V., Hodnik, V., Anderluh, G., Ban, N. & Weygand- Đurašević, I. (2014) Archaeal aminoacyl-tRNA synthetases interact with the ribosome to recycle tRNAs. Nucleic acids research, 42 (8), 5191-5201 doi:10.1093/nar/gku164.
@article{article, author = {Godini\'{c} Mikul\v{c}i\'{c}, Vlatka and Jari\'{c}, Jelena and Franke, Vedran and Hodnik, Vesna and Anderluh, Gregor and Ban, Nenad and Weygand- \DJura\v{s}evi\'{c}, Ivana}, year = {2014}, pages = {5191-5201}, DOI = {10.1093/nar/gku164}, keywords = {aminoacyl-tRNA synthetase, protein complex, ribosome, tRNA, SPR, thermophoresis}, journal = {Nucleic acids research}, doi = {10.1093/nar/gku164}, volume = {42}, number = {8}, issn = {0305-1048}, title = {Archaeal aminoacyl-tRNA synthetases interact with the ribosome to recycle tRNAs}, keyword = {aminoacyl-tRNA synthetase, protein complex, ribosome, tRNA, SPR, thermophoresis} }
@article{article, author = {Godini\'{c} Mikul\v{c}i\'{c}, Vlatka and Jari\'{c}, Jelena and Franke, Vedran and Hodnik, Vesna and Anderluh, Gregor and Ban, Nenad and Weygand- \DJura\v{s}evi\'{c}, Ivana}, year = {2014}, pages = {5191-5201}, DOI = {10.1093/nar/gku164}, keywords = {aminoacyl-tRNA synthetase, protein complex, ribosome, tRNA, SPR, thermophoresis}, journal = {Nucleic acids research}, doi = {10.1093/nar/gku164}, volume = {42}, number = {8}, issn = {0305-1048}, title = {Archaeal aminoacyl-tRNA synthetases interact with the ribosome to recycle tRNAs}, keyword = {aminoacyl-tRNA synthetase, protein complex, ribosome, tRNA, SPR, thermophoresis} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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