Naslov
Study on cell oxidation-reduction equilibrium after modulated radiofrequency radiation

Autori
Marjanović, Ana Marija ; Pavičić, Ivan ; Trošić, Ivančica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The Second International Conference on Radiation and Dosymetry in Various Fields of Research (RAD 2014) / - , 2014

Skup
International Conference on Radiation and Dosymetry in Various Fields of Research (2 ; 2014)

Mjesto i datum
Niš, Srbija, 27.05.2014. - 30.05.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
ROS ; in vitro ; RF

Sažetak
There has been a tremendous increase in the use of mobile phones in the past few decades and concerns are growing about possible hazardous effect of radiofrequency electromagnetic waves emitted by these devices on living organisms. The exact mechanism that could explain effects at non-thermal level is still unknown and under observation. One of the plausible explanations could be associated with oxidation-reduction imbalance which leads to the oxidative stress. Aim of this initial study was to evaluate the proposed mechanism. Modulated radiofrequency field of 1800 MHz, strength of 30 V/m was generated within Gigahertz Transversal Electromagnetic Mode cell (GTEM-cell) equipped by signal generator, modulator and amplifier. Continuous cell line V79, Chinese hamster lung fibroblasts, was irradiated with selected radiation for 10, 30 and 60 minutes. SAR was calculated to be 1.6 W/kg. We determined cell metabolic activity and viability by 3-(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide assay (MTT). Total protein content was determined by commonly used colorimetric method. Concentration of oxidised proteins was evaluated with an enzyme-linked immunosorbent assay (ELISA) which measures the protein carbonyl derivatives formed from protein oxidation. Reactive oxygen species (ROS) were marked with fluorescent probe 2′, 7′-dichlorofluorescin diacetate (DCFH-DA) and measured by means of plate reader device. In comparison to control cell samples metabolic activity and total protein content in exposed cells did not differ significantly. Concentrations of protein carbonyl in exposed cells insignificantly but continuously increase with duration of exposure. In exposed cell samples ROS level significantly increased after 10 min exposure. Decrease in ROS level was observed after 30 min treatment which indicates to the antioxidant defence mechanism activation. In conclusion under the given laboratory conditions, modulated radiofrequency radiation might cause impairment in cell oxidation-reduction equilibrium within the cells in culture.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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