Pregled bibliografske jedinice broj: 723895
Effect of 16S rRNA methyltransferase RmtD on biofilm formation and pyocyanin production in Pseudomonas aeruginosa PAO1
Effect of 16S rRNA methyltransferase RmtD on biofilm formation and pyocyanin production in Pseudomonas aeruginosa PAO1 // Book of Abstracts
Madrid, Španjolska, 2014. str. 211-211 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 723895 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Effect of 16S rRNA methyltransferase RmtD on biofilm formation and pyocyanin production in Pseudomonas aeruginosa PAO1
Autori
Vidučić, Darija ; Obranić, Sonja ; Maravić Vlahoviček, Gordana
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of Abstracts
/ - , 2014, 211-211
Skup
III. International Conference on Antimicrobial Research ICAR 2014
Mjesto i datum
Madrid, Španjolska, 01.10.2014. - 03.10.2014
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Pseudomonas aeruginosa PAO1; 16S rRNA methyltransferase RmtD; biofilm; pyocyanin
Sažetak
Pseudomonas aeruginosa is one of the leading causes of life-threatening nosocomial infections. The problem of antibiotic resistance in P. aeruginosa is highlighted by the emergence of multi-drug resistant strains. One of the pathogenic potential of P. aeruginosa is based on formation of biofilm during chronic infections. The regulation of many virulence associated factors, such as pyocyanin production and as well biofilm formation in P. aeruginosa, are under the control of quorum sensing system. RmtD is a methyltransferase that confers resistance to aminoglycosides, andwas identified in an P. aeruginosa clinical isolate. In this study, we investigated the link between 16S rRNA methyltransferase RmtD and biofilm formation and production of virulence factors in P. aeruginosa. Expression of RmtD in P. aeruginosa PAO1, demonstrated that RmtD positively affected biofilm formation in P. aeruginosa PAO1. Furthermore, linking its role in regulating virulence factors production such as pyocyanin, revealed that RmtD negatively affected the production of this virulent factor, regulated by quorum sensing system. Our further interest was to investigate the effect of RmtD on biofilm formation in P. aeruginosa mutants defective in production of RhlI/R quorum sensing system. We observed positive effect of RmtD on biofilm formation in these mutant strains. These results indicate that 16S rRNA methyltransferase RmtDexerts both positive and negative effect on quorum sensing system in P. aeruginosa PAO1 and further studies will focus on deciphering the link between 16S rRNA methyltransferase RmtD and quorum sensing.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Farmacija
POVEZANOST RADA
Projekti:
006-0982913-1219 - Molekularne osnove djelovanja antibiotika i mehanizmi bakterijske rezistencije (Maravić Vlahoviček, Gordana, MZOS ) ( CroRIS)
Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb