Naslov
Global histone acetylation in diabetic embryopathy

Autori
Korolija, Marina ; Hadžija, Mirko ; Sobočanec, Sandra ; Popović Hadžija, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
The interpalay of biomolecules ; Congres of the Croatian society of biochemistry and molecular biology

Mjesto i datum
Zadar, Hrvatska, 24.09.2014. - 27.09.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
diabetic embryopathy; neural tube defects; histone acetylation

Sažetak
Maternal diabetes increases the rate of congenital malformations in embryos, causing diabetic embryopathy which mostly affects heart and neural tube (embryonic precursor of central nervous system) in both humans and animal models. High maternal blood glucose concentration, which is presumably the major teratogenic factor, obstructs the process of embryonic neurulation (neural tube closure) by an unknown mechanism. Resulting open NTDs (neural tube defects) arise along rostrocaudal axis of the tube, affecting brain (exencephaly), spine (spina bifida aperta) or the entire neural tube (craniorachischisis). It has been shown that embryos developing in hyperglycemic milieu display disrupted gene expression pattern which seems rather non- specific, varying markedly between different mouse strains, or even between individual organisms of the same inbred strain. In an attempt to approach this issue from the point of genome-wide gene regulation, we analyzed global histone H3 and histone H4 acetylaton in 44 non-obese diabetic (NOD) mouse embryo specimens at midgestation (embryonic day 10.5). Hypoacetilation of both H3 and H4 has been detected in embryos with spina bifida aperta and craniorachischisis, but not exencephaly. Further, more detailed analysis of histone acetylation at gene promoters is needed to establish potential relationship between histone acetylation and transcriptional regulation in diabetic embryopathy.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA