Naslov
THE EVALUATION OF NEUROVIRULENCE OF MUMPS VIRUS STRAINS WITH ALTERNATIVE NEWBORN RAT-BASED SAFETY TEST

Autori
Lang Balija, Maja ; Šantak, Maja ; Markušić, Maja ; Forčić, Dubravko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka 2. simpozija Hrvatskog društva za znanost o laboratorijskim životinjama "Pokusne životinje u znanstvenim istraživanjima" / Dubravka Švob Štrac, Maja Lang Balija, Julija Erhardt, Jadranka Bubić Špoljar, Blanka Smolić - Zagreb : Hrvatsko društvo za znanost o laboratorijskim živoitnjama, 2014, 66-67

Skup
2. simpozija Hrvatskog društva za znanost o laboratorijskim životinjama "Pokusne životinje u znanstvenim istraživanjima"

Mjesto i datum
Zagreb, Hrvatska, 10.10.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
rat-based neurovirulence safety test; mumps virus

Sažetak
Because of neurotropic and neurovirulent properties of mumps virus, neurovirulence testing of live attenuated vaccine is required by most national regulatory organisations. Such testing is preformed in monkeys (Ph.Eu. 01/2008:20618-Test for neurovirulence of live virus vaccines). But results obtained from these tests do not necessarily distinguish among the neurovirulent strain from those that are not. As a part of an international collaborative study we investigated the neurovirulence of three vaccine strains (JL5, Urabe AM9 and L-Zagreb) and two wild–type mumps viruses isolated in Croatia (9218/Zg98 and MuVi/Zagreb.HRV/28.12) by the neurovirulence assay in newborn rats. The results obtained by alternative assay on two vaccine strains (JL5 and Urabe AM9) correspond to the results obtained by the National Institute for Biological Standards (NIBSC) in the United Kingdom and the Food and Drug Administration in the United Station. Strain L-Zagreb also showed reduced neurovirulent properties as expected because it is a vaccine strain. Wild-type mumps virus isolates showed high neurovirulence. These results indicate as that the test in newborn rats is suitable for assessing the neurovirulence of mumps viruses. The test is robust, reproducible and follows the 3R principles.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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