Naslov
Role of flavonoids on oxidative stress and mineral contents in the retinoic acid-induced bone loss model of rat

Autori
Oršolić, Nada ; Goluža, Eleonora ; Đikić, Domagoj ; Lisičić, Duje ; Jeleč, Željko ; Vihnanek Lazarus, Maja ; Orct, Tatjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Drugi simpozij Hrvatskoga društva za znanost o laboratorijskim životinjama s međunarodnim sudjelovanjem „Pokusne životinje u znanstvenim istraživanjima“ : Knjiga sažetaka = 2nd Symposium of Croatian Laboratory Animal Science Association with international participation “Experimental animals in scientific research” : Book of Abstracts / - Zagreb, 2014, 80-81

Skup
Drugi Simpozij Hrvatskoga društva za znanost o laboratorijskim životinjama s međunarodnim sudjelovanjem „Pokusne životinje u znanstvenim istraživanjima“ (2 ; 2014)

Mjesto i datum
Zagreb, Hrvatska, 10.10.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
flavonoids; retinoic acid; bone loss prevention; oxidative stress; rat

Sažetak
Reactive oxygen species (ROS) play a role in a number of degenerative conditions including osteoporosis. Flavonoids as phytoestrogens exert physiological effects against oxidative stress diseases. We developed a retinoic acid-induced bone loss model (RBL) of rats to assess whether flavonoids and alendronate as positive control have role against oxidative stress and mineral contents in osteoporosis in vivo. Methods Three month-old female rats of the Y59 strain were given quercetin, chrysin, naringenin (100 mg kg-1) or alendronate (40 mg kg-1, a positive control) immediately before retinoic acid treatment (80 mg kg-1) once daily for 14 days by a single intragastric (i.g.) application. In the second part of the study, we assessed the effect of those flavonoids on the skeletal system of healthy rats using single i.g. application on the respective flavonoids during 14 days. Twenty four hours after the treatment, we analysed bone mineral density (BMD) and the total content of bone calcium and phosphorus in the femur, the geometric and physical characteristics of thigh bones and lipid peroxidation and glutathione levels of liver and kidney cells. Results All flavonoids improved the decrease in bone weight coefficient, the length and the diameter of the bone, the content of bone ash and calcium and phosphorus content induced by retinoic acid. Chrysin and quercetin showed promise as preventive agents. Flavonoids were superior to alendronate according to some criteria. These results suggest that the dietary flavonoids could reduce retinoic acid-induced oxidative stress and bone loss and that flavonoids may be useful therapeutics for prevention of skeletal diseases.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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