Naslov
EXPRESSION OF SODIUM-D-GLUCOSE COTRANSPORTER 1 IN MURINE TISSUES ; SEX-DEPENDENT EXPRESSION IN KIDNEYS

Autori
Vrhovac, Ivana ; Breljak, Davorka ; Karaica, Dean ; Koepsell, Hermann ; Sabolic, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Experimental animals in scientific research / Hrvatsko društvo za znanost o laboratorijskim životinjama - Zagreb, 2014, 110-111

Skup
2nd Symposium of Croatian Laboratory Animal Science Assiociation with international participation

Mjesto i datum
Zagreb, Hrvatska, 10.10.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
intestine; glucose transporters; mouse organs; proximal tubules; sex differences; SGLT1

Sažetak
Sodium-D-glucose cotransporter SGLT1 (SLC5A1) in the brush-border membrane (BBM) of small intestinal enterocytes and renal proximal tubule (PT) epithelium is responsible for glucose absorption and reabsorption, respectively. Detailed localization of SGLT1 in other mammalian organs is poorly known. Since mice are frequently used experimental animals in preclinical testing, it is important to determine the expression of mouse Sglt1 (mSglt1) in their organs/tissues. To investigate the mSglt1 protein expression in different organs/tissues by immunocytochemistry (IC) in tissue cryosections and by Western blotting (WB) in isolated cell membranes, we used the adult wild-type (WT) and Sglt1 knock-out (KO) mice of both sexes. Specificity of the polyclonal mSglt1-antibody was confirmed in Sglt1 KO mice ; in WT mice, by WB in the jejunal and renal BBM the antibody labeled a single protein band of 75-kDa, whereas in the Sglt1 KO mice membranes, these protein bands were absent. The IC studies in the kidneys of WT mice revealed localization of the protein in PT BBM, exhibiting segmental (S2>S3), zonal (cortex>outer stripe) and sex (males>females) differences in staining intensity, and in the apical membrane of thick ascending limb of Henle and macula densa. However, contrary to the male-dominant protein expression, the renal Sglt1 mRNA expression, analyzed by qPCR, was female-dominant. The mSglt1-antibody further stained the luminal domain of bile and pancreatic ducts, whereas the spleen, cerebrum, cerebellum, fat and skeletal muscle, remained unstained. The observed distribution of mSglt1 protein in various mouse organs will enable further studies of its role in patho/physiological, pharmacological and toxicological conditions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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