Pregled bibliografske jedinice broj: 719861
Genetic characterization of hepadnaviruses associated with histopathological changes in the liver of duck and goose embryos
Genetic characterization of hepadnaviruses associated with histopathological changes in the liver of duck and goose embryos // Veterinary microbiology, 174 (2014), 3/4; 302-308 doi:10.1016/j.vetmic.2014.09.026 (međunarodna recenzija, članak, znanstveni)
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Naslov
Genetic characterization of hepadnaviruses associated with histopathological changes in the liver of duck and goose embryos
Autori
Biđin, Marina ; Tišljar, Marina ; Biđin, Zdenko ; Lojkić, Ivana ; Majnarić, Darko
Izvornik
Veterinary microbiology (0378-1135) 174
(2014), 3/4;
302-308
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
duck; goose; embryos; hepadnavirus; S-protein gene; histopathology
Sažetak
Avian hepadnaviruses are aetiological agents of hepatitis B, that has been identified primarily in ducks, and more recently in various avian species. In this paper, 16 hepadnaviruses were detected by polymerase chain reaction (PCR) in the field samples from dead embryos of commercially reared domestic duck and goose. Based on the molecular analysis of the S-protein gene sequences and phylogenetic Neighbour-joining tree, identified viruses were clustered in the same genetic group, indicating no host-related diversity. Both duck and goose-origin hepadnaviruses were grouped within the cluster consisting of ''Western-country'' and ''Chinese'' duck hepatitis B (DHBV) isolates, showing more evolutionary distances with other known avian hepadnaviruses. Histopathologically, the lesions observed in the liver tissue from hepadnavirus positive duck and goose embryos varied from low to mild degree of perivascular mononuclear cells and mixtocellular infiltrations, followed by mild vacuolar changes. Small focal necrotic changes in the liver parenchyma, and bile ductular proliferation were also found in examined liver samples. Generally, the microscopic findings resemble those described in experimentally infected ducks, while this was the first description of hepadnavirus associated lesions in domestic goose. Although hepadnaviruses are considered to have a very narrow host range, this study showed that domestic ducks and geese are susceptible to infection with genetically almost identical hepadnaviruses, that were likely to produce similar microsopic changes in the liver of both duck and goose embryos. The impact of naturally occurred hepadnavirus infection and possible synergistic interactions with other infectious or non-infectious agents on embryo viability needs futher investigation.
Izvorni jezik
Engleski
Znanstvena područja
Veterinarska medicina
POVEZANOST RADA
Projekti:
048-0531863-0464 - Uloga dušik (II) oksida u bolestima kardiovaskulanog sustava peradi (Tišljar, Marina, MZOS ) ( CroRIS)
053-0531863-1856 - Imunosupresivni i crijevni virusi peradi u Hrvatskoj (Biđin, Zdenko, MZOS ) ( CroRIS)
Ustanove:
Hrvatski veterinarski institut, Zagreb,
Veterinarski fakultet, Zagreb
Profili:
Darko Majnarić
(autor)
Marina Biđin
(autor)
Zdenko Biđin
(autor)
Marina Tišljar
(autor)
Ivana Lojkić
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE