Naslov
Adsorption of Proteins and Cell Adhesion to Plasma Treated Polymer Substrates

Autori
Recek, Nina ; Mozetič, Miran ; Jaganjac, Morana ; Milković, Lidija ; Žarković, Neven ; Vesel, Alenka

Izvornik
International journal of polymeric materials (0091-4037) 63 (2014), 13; 685-691

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
biocompatibility ; cell adhesion ; oxygen and fluorine plasma treatment ; polymer surface modification ; protein adhesion

Sažetak
Plasma treatment is often used to alter cell interaction with polymer surfaces used in biomedical application. The influence of surface hydrophilicity/hydrophobicity on human mammary epithelial cell (HMEC) proliferation and adhesion of protein albumin to plasma treated polystyrene (PS) was studied. The PS surface was made hydrophilic or hydrophobic by treatment either in O2 or CF4 plasma. The rate of protein adhesion was studied by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) after incubation of PS in albumin solution for different periods, while cell viability and morphology was studied by MTT assay and scanning electron microscope (SEM). XPS measurements have shown that the quantity of adsorbed protein was higher for both plasma treated samples than for the untreated one. No significant difference regarding protein adhesion on hydrophilic or hydrophobic plasma treated surface was found by XPS. Contrary, the results for cell proliferation showed much better proliferation on hydrophilic surface.

Izvorni jezik
Engleski

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