Pregled bibliografske jedinice broj: 718192
Intracellular nanosurgery and cell enucleation using a picosecond laser
Intracellular nanosurgery and cell enucleation using a picosecond laser // Journal of microscopy, 234 (2009), 1; 1-8 doi:10.1111/j.1365-2818.2009.03142.x (međunarodna recenzija, članak, znanstveni)
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Naslov
Intracellular nanosurgery and cell enucleation using a picosecond laser
Autori
Raabe, Isabel ; Vogel, Sven ; Peychl, Jan ; Tolić- Norrelykke, Iva Marija
Izvornik
Journal of microscopy (0022-2720) 234
(2009), 1;
1-8
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
intracellular nanosurgery
Sažetak
Living cells are highly organized in space and time, which makes spatially and temporally confined manipulations an indispensable tool in cell biology. Laser-based nanosurgery is an elegant method that allows precise ablation of intracellular structures. Here, we show cutting of fluorescently labelled microtubules and mitotic spindles in fission yeast, performed with a picosecond laser coupled to a confocal microscope. Diverse effects from photo- bleaching to partial and complete breakage are obtained by varying the exposure time, while simultaneously imaging the structures of interest. Using this system we developed an efficient technique to generate enucleated cells without perturbing the distribution of other organelles. This enucleation method can be used to study the cytoskeleton in a nucleus- free environment, as well as the role of the nucleus in cell growth and a variety of cellular functions.
Izvorni jezik
Engleski
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
