Naslov
Comparison of in vitro toxicity of silver ions and silver nanoparticles on human hepatoma cells

Autori
Vinković Vrček, Ivana ; Žuntar, Irena ; Petlevski, Roberta ; Pavičić, Ivan ; Dutour Sikirić, Maja ; Ćurlin, Marija ; Goessler, Walter

Izvornik
Environmental toxicology (1520-4081) 31 (2016); 679-692

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
silver nanoparticles; ionic silver; HepG2 cells; cytotoxicity; oxidative stress

Sažetak
The scientific information on potential harmful effects of silver nanoparticles (AgNPs) on human health severely lags behind their exponentially growing applications in consumer products. Recent investigation on the absorption behaviour of coated AgNPs has showed that silver can pass through the full thickness human skin. Upon reaching the blood circulation, it can be further distributed to the other organs, or become metabolized in the liver and kidney. In assessing toxic risk of AgNP usage, liver, as a detoxifying organ, becomes particularly important. In order to provide a basic knowledge on consequences of exposure of the liver organ to AgNP, this study is aimed to evaluate cytotoxicity parameters and antioxidative response induction in human hepatoblastoma (HepG2) cells treated with nano and ionic form of silver. Our research showed that citrate-coated silver nanoparticles could enter the liver cells, induce cytotoxicity, and cause oxidative stress. The LDH leakage, inhibition of albumin synthesis along with decreased ALT activities indicated that the treatment with AgNP resulted in membrane damage and reduced cell function of human liver cells. Observed cytotoxic effect of AgNP on HepG2 cells was substantially weaker than that caused by ionic silver, while the uptake of nano and ionic form of silver by the HepG2 cells was nearly the same.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA