Naslov
Accelerated inactivation of cardiac L-type calcium channels triggered by anaesthetic-induced preconditioning

Autori
Tampo, A. ; Hogan, C.S. ; Sedlić, Filip ; Bošnjak, Z.J. ; Kwok, W.M.

Izvornik
British journal of pharmacology (0007-1188) 156 (2009), 3; 432-443

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
preconditioning; calcium channel; isoflurane; cardioprotection

Sažetak
Cardioprotection against ischaemia by anaesthetic-induced preconditioning (APC) is well established. However, the mechanism underlying Ca(2+) overload attenuation by APC is unknown. The effects of APC by isoflurane on the cardiac L-type Ca channel were investigated. In a model of in vivo APC, Wistar rats were exposed to isoflurane (1.4%), delivered via a vaporizer in an enclosure, prior to thoracotomy. The Dahl S rats were similarly preconditioned to determine strain-dependent effects. Whole-cell patch clamp using cardiac ventricular myocytes was used to determine the L-type Ca(2+) current (I(Ca, L)) characteristics and calmodulin (CaM) levels were determined by Western blot analysis. Cytosolic Ca(2+) levels were monitored using fluo-4-AM. Action potential (AP) simulations examined the effects of APC. In Wistar rats, APC significantly accelerated I(Ca, L) inactivation kinetics. This was abolished when external Ca(2+) was replaced with Ba(2+), suggesting that Ca(2+)-dependent inactivation of I(Ca, L) was modulated by APC. Expression levels of CaM, a determinant of I(Ca, L) inactivation, were not affected. Attenuation of cytosolic Ca(2+) accumulation following oxidative stress was observed in the APC group. Simulations showed that the accelerated inactivation of I(Ca, L) resulted in a shortening of the AP duration. The Dahl S rat strain was resistant to APC and changes in I(Ca, L) inactivation were not observed in cardiomyocytes prepared from these rats. APC triggered persistent changes in the inactivation of cardiac L-type Ca channels. This can potentially lead to a reduction in Ca(2+) influx and attenuation of Ca(2+) overload during ischaemia/reperfusion.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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