Naslov
Binding of a new phosphonium dye with AT polynucleotide studied by SERS

Autori
Dijanošić, Adriana ; Miljanić, Snežana ; Novak, Morana ; Lesev, Nedyalko ; Vasilev, Aleksey ; Deligeorgiev, Todor ; Crnolatac, Ivo ; Piantanida, Ivo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XXIV. International Conference on Raman Spectroscopy / Opfer ; Britta ; Siegesmund, Daniel - Jena : Leibniz Institute of Photonic Technology, 2014, 176-176

Skup
XXIV. International Conference on Raman Spectroscopy

Mjesto i datum
Jena, Njemačka, 10.08.2014. - 15.08.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
surface-enhanced Raman scattering; phosphonium dye; polynucleotide

Sažetak
Surface-enhanced Raman scattering (SERS) of a new phosphonium dye was studied on a citrate-reduced silver colloid upon NIR excitation. Differences in relative band intensities between spectra were attributed to concentration dependent variations in position of the dye molecules on the silver surface, whereby at high concentration intermolecular stacking was suggested. Known for its ability to interact with DNA, SERS spectra of the dye with alternating adenine-thymine polynucleotide were acquired. Although all studied dye/poly dAdT-poly dAdT mixtures contained the same amount of the dye, at excess of polynucleotide the spectrum of the complex resembled the spectrum of the dye at low concentration, while excess of the dye over biopolymer produced the spectrum similar to that at high dye concentration. Given that excess of the dye induces formation of dimer while binding with AT polynucleotide, observed spectra could be indicative of the dye assembly within the polynucleotide groove.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA