Naslov
Determination of anthocyanins in hybrid wine by HPLC-DAD AND HPLC-MS

Autori
Tomaz, Ivana ; Maslov, Luna ; Karoglan Kontić, Jasminka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / - Zagreb, 2012

Skup
13th International Chromatography School

Mjesto i datum
Zagreb, Hrvatska, 18.06.2012. - 19.06.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
hybrid wines ; anthocyanins ; HPLC ; mass spectrometer

Sažetak
Anthocyanins are a family of polyphenols that are directly responsible for colour in grapes and wines. The anthocyanins profile of a grape and its wine, determined by the relations of the different anthocyanins, is characteristic of each variety. The hybrid grapes include anthocyanins monoglucosides and diglucosides. The objective of this work is to determinate and identify anthocyanin profile in hybride wines from Cabernet Cortis, vintage 2009. Wine samples were directly injected to HPLC with diode array detector. Identification was ca rried out by comparing spectra and retention times with standards. The eluation order was confirmed by HPLC - MS analysis. In this experiment reversed phase column (Luna Phenyl - Hexyl, 250 x 4, 6 mm, i.d. 5 μ m, Phenomen ex, USA) with gradient eluation and UV detection at 518 nm was used. The solvents were (A) aqueous 0, 1% formic acid, and (B) acetonitril containing 0, 1% form ic acid. Mas s spectrometry (MS) was done by electrospray ionisation in positive ion mode. Obtain mass spectras were compared with this one find in the literature.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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