Naslov
Characterization of Environmental CTX-M-15- Producing Stenotrophomonas maltophilia

Autori
Maravić, Ana ; Skočibušić, Mirjana ; Fredotović, Željana ; Cvjetan, Svjetlana ; Šamanić, Ivica ; Puizina, Jasna

Izvornik
Antimicrobial agents and chemotherapy (0066-4804) 58 (2014), 10; 6333-6334

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pismo, znanstveni

Ključne riječi
Stenotrophomonas maltophilia ; CTX-M-15 ; ESBL ; SXT ; environment

Sažetak
Stenotrophomonas maltophilia is a widespread environmental microorganism that has emerged as a significant opportunistic pathogen due to its intrinsic resistance to almost all available antibiotics. Forty-one S. maltophilia isolates were recovered from retail shellfish (Mytilus galloprovincialis ; 8 isolates) purchased at fish markets in Split, Croatia, and from coastal marine waters near Split (33 isolates) in 2012. Strains were isolated on imipenem (16 µg/ml)-containing tryptic soy agar at 30°C and identified using API 20NE. Nineteen isolates carried blaCTX-M-15, six of which additionally harbored blaTEM-116, and one carried blaTEM- 127. S1 nuclease pulsed-field gel electrophoresis (PFGE) of plasmid DNA followed by Southern blotting showed that blaCTX-M-15 was located on large plasmids of various sizes. Interestingly, all these plasmids belonged to the IncFIB incompatibility group. PFGE of XbaI- digested genomic DNA showed heterogeneity among CTX-M-15-producing isolates. The isolates were also investigated for class 1, 2, and 3 integrases, gene cassettes, and the sul1 and sul2 genes by PCR. This report presents the first description of IncF::CTX-M-15 in S. maltophilia isolates of environmental and clinical origins and of SXT resistance traits previously found only in clinical isolates, emphasizing the possibility that S. maltophilia isolates are hidden reservoirs for these multidrug resistance determinants.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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