Naslov
First phylogenetic analysis of pseudorabies virus in domestic pigs and wild boars in Croatia

Autori
Keros, Tomislav ; Brnić, Dragan ; Prpić, Jelena ; Dežđek, Danko ; Jemeršić, Lorena ; Roić, Besi ; Bedeković, Tomislav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
11th EWDA Conference / Anna Meredith - Edinburgh : The Royal (Dick) School of Veterinary Studies The University of Edinburgh, 2014

Skup
EWDA Conference 2014

Mjesto i datum
Edinburgh, Ujedinjeno Kraljevstvo, 25.08.2014. - 30.08.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
PRV; pigs; wild boars; Croatia

Sažetak
Even though serological data implies that Pseudorabies is present in domestic pigs and wild boars in Croatia, no comprehensive virological survey has yet been carried out. Therefore, this study included testing of brain tissue samples collected from 200 domestic pigs and 105 samples of wild boar originating from seventeen districts in Croatia. For the detection of gB-gene of Pseudorabies virus (PRV) DNA a nested PCR protocol was carried out. The presence of PRV DNA was confirmed in samples originating from six domestic pigs (3.0%) and one wild boar (0, 95%).Positive samples were sequenced and analyzed on the basic of gC genome fragment. Croatian PRV strains have shown to be genetically identical and they share strong relatedness with some representative strains in relatively heterogeneous Clade A. This is the first phylogenetic characterization of PRV strains detected in domestic pigs and wild boars in Croatia, as a result of a larger epidemiological survey. The results clearly show that PRV is still circulating among domestic pig population in Croatia. Furthermore, the presence of pseudorabies virus in wild boars underlines the importance of this species as PRV reservoirs. The continued surveillance is necessary to track the viral spread which may lead to final eradication of the disease.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA