Naslov
Molecular mobility of scaffolds’ biopolymers influences cell growth

Autori
Podlipec, Rok ; Gorgieva, Selestina ; Jurašin, Darija ; Urbančič, Iztok ; Kokol, Vanja ; Štrancar, Janez

Izvornik
ACS Applied materials and interfaces (1944-8244) 6 (2014), 18; 15980-15990

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Scaffold biocompatibility; polymer mobility; morphology; viscoelasticity; cell growth

Sažetak
Understanding biocompatibility of materials and scaffolds is one of the main challenges in the field of tissue engineering and regeneration. Complex nature of cell-biomaterial interaction requires extensive preclinical functionality testing by studying specific cell responses to different biomaterial properties, from morphology and mechanics to surface characteristics at the molecular level. Despite constant improvements, a more general picture of biocompatibility is still lacking and tailor-made scaffolds are not yet available. The scope of our study was thus the investigation of the correlation of fibroblast cell growth on different gelatin scaffolds with their morphological, mechanical as well as surface molecular properties. The latter were thoroughly investigated via polymer molecular mobility studied by site-directed spin labelling and electron paramagnetic resonance spectroscopy (EPR) for the first time. Anisotropy of the rotational motion of the gelatin side chain mobility was identified as the most correlated quantity with cell growth in the first days after adhesion, while weaker correlations were found with scaffold viscoelasticity and no correlations with scaffold morphology. Namely, the scaffolds with highly mobile or unrestricted polymers identified with the cell growth being five times less efficient (Ncells = 60 ± 25 mm-2) as compared to cell growth on the scaffolds with considerable part of polymers with the restricted rotational motion (Ncells = 290 ± 25 mm-2). This suggests that molecular mobility of scaffold components could play an important role in cell response to medical device, reflecting new aspect of biocompatibility concept.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Farmacija

POVEZANOST RADA