Naslov
TNFα gene/protein in tumorigenesis of sporadic colon adenocarcinoma

Autori
Kapitanović, Sanja ; Čačev, Tamara ; Catela Ivković Tina ; Lončar, Božo ; Aralica, Gorana

Izvornik
Experimental and molecular pathology (0014-4800) 97 (2014), 2; 285-291

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
TNFα; colon cancer; SNPs; mRNA; immunohistochemistry

Sažetak
Inherited polymorphisms in immunomodulatory genes such as cytokines may contribute to variation in immunological response and genetic susceptibility for complex diseases, including cancer. TNFα can mediate tumor progression by inducing proliferation, invasion and metastasis of tumor cells. The aim of our study was to examine allelic frequencies of TNFα promoter SNPs, -1031 T/C, -857 C/T, -308 G/A and -238 G/A, in patients with sporadic colon adenocarcinoma in order to investigate the possible role of these SNPs on susceptibility to sporadic colon cancer. Another aim of this study was to examine the influence of TNFα SNPs on TNFα mRNA and protein expression in colon tumors and their possible role in development and progression of this type of tumor. The distribution of genotypes for all four TNFα SNPs in patients showed no significant difference compared to controls. No statistically significant difference in TNFα mRNA expression in tumors and corresponding normal mucous tissue was found (p=0.14). Statistically significant (p=0.028) difference was found between TNFα mRNA expression in histological grade 1 in comparison to histological grade 2 and 3 of tumors. Additionally, statistically significant correlation (p=0.03) was found between TNFα -857 C/T genotypes and TNFα mRNA expression in tumor tissue. TNFα mRNA expression was significantly higher in tumor tissue of patients with -857 CT and -857 TT genotype. Most of the tumors (78.26%) were positive for TNFα protein. No correlation was found between the TNFα protein expression and clinicopathological characteristics as well as with TNFα genotypes. However, patients with TNFα protein negative tumors had longer survival but the result was not statistically significant (p=0.365). Conclusion: Our results suggest the role of TNFα as one of the immunomodulatory genes in progression of sporadic colon cancer.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA