Naslov
Movement and Sequestration of Inert Particles in the Rat Lungs after Microwave Irradiation

Autori
Trošić, Ivančica ; Mataušić-Pišl, Mirjana ; Radalj, Željko ; Bušljeta, Ivana.

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Crucial Issues in Inhalation Research - Mechanistic, Clinical and Epidemiologic, 8th International Inhalation Symposium (INIS) / Heinrich, Uwe - Hannover : MHH Press, 2001, 73-73

Skup
8th International Inhalation Symposium (INIS)

Mjesto i datum
Hannover, Njemačka, 06.06.2001. - 09.06.2001

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
microwave irradiation; particles; rat lung free cells; movement

Sažetak
The effect of microwave radiation on inert particle movement and sequestration within the lungs was investigated in rats. The test material were biologically inert particles (Fluorescent carboxylated polystyrene microspheres 2.37 ľm dia., Polyscience Inc., Warrington, PA) instilled to healthy, male Wistar rats (N=40), twenty of which were then exposed to microwaves (2450 MHz, 5-15mV/cm2), 2 h/day, 5 days/week for 30 days. The movement and the sequestration of particles were assessed by measurement of alveolar, lung, and lymphonodal burden in animals sacrificed after 4, 14, 26, and 44 hours of the microwave exposure. Alveolar cells obtained by the lung lavage were determined quantitatively and qualitatively. Lung and lymph tissues were chemically dissolved, passed through Nuclepore filters and the tissue burdens were determined using a fluorescent microscope. Compared to controls, the irradiated group of animals showed a slight time-dependent decrease in the free lung cell population. There were no significant differences in cell viability between the exposed and non-exposed groups. Throughout the experiment, the macrophage activity in the treated animals was slightly decreased. The irradiated animals showed significantly lower burdens of microspheres in the lung compartments than non-irradiated animals. A lower quantity of microspheres was retrieved from the alveolar space of irradiated animals than from controls, including the first day of the experiment. The microsphere burden deposited in the lung tissue was decreased in treated than in non-treated rats. A slight time-dependent increase was noted in the irradiated group after eight experimental day. Opposite to the control, the irradiated group showed an increasing tendency to accumulate particles in the lymph nodes. To conclude, microwaves seem to affect the early clearance, i.e. the ciliary excalator. It is likely that some particles are cleared through mucociliary clearance within the twenty-four hours after deposition in the alveolar space of microwave-treated rats. The particles retained in the lungs were available for transport to lung-associated lymph nodes.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

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