Naslov
Effect of high salt diet on expression of antioxidative enzymes, hypoxia inducible factor 1 alpha and cyclooxygenase 1 and 2 in blood vessels of Sprague-Dawley rats

Autori
Ćosić, Anita ; Novak, Sanja ; Mihalj, Martina ; Mihaljević, Zrinka ; Čavka, Ana ; Grizelj, Ivana ; Drenjančević, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of The Physiological Society 31 / - , 2014

Skup
Physiology 2014

Mjesto i datum
London, Ujedinjeno Kraljevstvo, 30.06.2014. - 02.07.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
high salt diet; antioxidative enzymes; hypoxia inducible factor 1; cyclooxygenase 1 and 2; blood vessels

Sažetak
Low-dose angiotensin II (ANG II) infusion restores impaired vascular relaxation responses to acetylcholine and hypoxia in high salt fed rats and increases Cu/Zn SOD expression in genetically modified HS fed rats. Decreased Cu/Zn- and Mn- superoxide dismutase protein expression in cerebral vessels of Dahl Salt-Sensitive rats is associated with increased oxidative stress and impaired vasodilator responses mediated via cyclooxygenases (COX). The aim of this study was to assess the effects of 1 week high salt diet (HS ; 4% NaCl) on antioxidative enzymes (Cu/Zn SOD, MnSOD, EC-SOD, catalase (CAT) and glutathione- peroxidase 4 (GPx4)), cyclooxygenase 1 and 2 (COX 1, 2) and hypoxia inducible factor 1 alpha (HIF-1α) gene expression in blood vessels. 11-weeks old healthy male Sprague-Dawley rats (N=14) were divided into control group fed with standard rat chow (0.4% NaCl) and HS group fed (4% NaCl) for 1 week. Following diet protocol, rats were anesthetized with ketamine (75 mg/kg) and midazolam (2.5 mg/kg), decapitated and their aorta and brain blood vessels harvested. Superoxide dismutase isoforms (Cu/Zn SOD, MnSOD, EC-SOD), HIF1α, COX 1, 2, GPx4, and catalase mRNA levels were determined by quantitative real-time PCR. Data were analyzed using SigmaPlot 11.0 and p<0.05 was considered significant. All experimental procedures conformed to the EU Guidelines for the Care and Use of Laboratory Animals (directive 86/609) and were approved by the local Ethical CommitteeRESULTS: Significantly higher expression of HIF-1α (p=0.002), COX1 (p=0.002), COX2 (p=0.002), GPX4 (p=0.015), Cu/Zn SOD (p=0.018), MnSOD (p=0.001) and EC-SOD (p=0, 001) were in aortas compared to cerebral vessels of control rats. HS rats had significantly down-regulated HIF-1α in aortas (p=0.037) and COX2 in cerebral blood vessels (p = 0.011) compared to controls. Furthermore, HS group had significantly decreased mRNA level of GPx4 (p=0.012) and Cu/Zn SOD (p=0.009), and increased level of COX1 (p=0.030) in aortas compared to aortas of controls. Cu/Zn SOD was significantly decreased (p=0, 009) in aorta of HS rats compared to controls. Catalase, EC-SOD and MnSOD gene expression was reduced in HS rats but did not reach statistical significance (p=0.054, p=0.055 and p=0.069, respectively) HS diet down-regulated HIF-1α and COX2 gene in aortas and cerebral blood vessels. All antioxidative enzymes genes were down-regulated by HS. These results suggest that HS may promote oxidative stress by reducing antioxidative capacity through HIF-1α and antioxidative gene expression modulation. In addition, there is altered expression of genes important in mediating functional vascular responses (i.e. COX1, 2) by HS diet.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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