Naslov
Acute application of antioxidants protects against hyperoxia-induced reduction of plasma nitrite concentration

Autori
Vučinović, Zoran ; Duplančić, Darko ; Šešelja Perišin, Ana ; Kukoč Modun, Lea ; Gunjača, Grgo ; Radman, Maja ; Vuković, Jonatan ; Tsikas, Dimitrios ; Poljak, Kolja ; Modun, Darko

Izvornik
Clinical physiology and functional imaging (1475-0961) 35 (2015), 1; 76-80

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kratko priopcenje, znanstveni

Ključne riječi
arterial stiffness; nitric oxide; oxygen; vasoconstriction; vitamin C

Sažetak
We investigated the effects of acute intake of antioxidants on hyperoxia-induced oxidative stress, reduction of plasma nitrite and change in arterial stiffness. Twelve healthy males randomly consumed either placebo or an oral antioxidant cocktail (vitamin C, 1000 mg ; vitamin E, 600 IU ; alpha-lipoic acid, 600 mg). Every therapy was consumed once, a week apart, in a cross-over design, 30 min before the experiment. The volunteers breathed 100% normobaric oxygen between 30th and 60th min of 1-h study protocol. Plasma levels of nitrite, lipid peroxides (LOOH) and vitamin C, arterial stiffness (indicated by augmentation index, AIx) and arterial oxygen (PtcO2) pressure were measured before and after hyperoxia. Exposure to oxygen caused a similar increase of PtcO2 in both placebo and antioxidants groups, confirming comparable exposure to hyperoxia (438 ± 100 versus 455 ± 83 mm Hg). Vitamin C was increased in the antioxidants group confirming successful application of antioxidants (69 ± 14 versus 57 ± 15 μm). Hyperoxia resulted in increased AIx and LOOH and decreased nitrite in placebo (−32 ± 11 versus −47 ± 13%, 72 ± 7 versus 62 ± 6 μm H2O2 and 758 ± 184 versus 920 ± 191 nm, respectively), but not in the antioxidants group (−42 ± 13 versus −50 ± 13%, 64 ± 9 versus 61 ± 8 μm H2O2 and 847 ± 156 versus 936 ± 201 nm, respectively). The acute intake of selected antioxidants was effective in preserving bioavailabity of ˙NO and vascular function, against hyperoxia-induced oxidative stress.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

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