Naslov
Quercetin induced DNA damage in human bladder cancer cells

Autori
Gajski, Goran ; Garaj-Vrhovac, Vera ; Oršolić, Nada

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Reviews of clinical pharmacology and drug therapy - Abstracts Book of the 18th International Congress Phytopharm 2014 / - Sankt Peterburg, 2014, 19-19

Skup
18th International Congress "Phytopharm 2014"

Mjesto i datum
Sankt Peterburg, Ruska Federacija, 03.07.2014. - 05.07.2014

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Quercetin; Comet assay; DNA damage; human bladder cancer cells

Sažetak
Quercetin is a typical plant flavonoid which possesses diverse pharmacologic effects including anti-inflammatory, anti-oxidant, anti-cancer, anti-anaphylaxis and anti-aging effects. Present study aimed to investigate possible cytotoxic and genotoxic effects of quercetin against human bladder cancer T24/83 cells in vitro. Quercetin in concentrations of 1 to 50 μM displayed dose dependent cytotoxicity in human bladder cancer T24/83 cells, as evaluated using MTT test. Furthermore, DNA damaging potential of quercetin was evaluated using the alkaline comet assay. For the evaluation of possible genotoxic effect of quercetin, T24/83 cells were treated with quercetinin concentrations of 1 and 50 µM for 2 h. After quercetin treatment all comet assay parameters (tail length, tail intensity and tail moment) showed statistically significant (P<0.05) increase for both concentrations compared to un-treated control. Tail length and tail moment parameters also showed significant (P<0.05) difference between quercetin treatments indicating that the observed effect was dose-dependent. Moreover, cells were classified as either ‘‘undamaged’’ or ‘‘damaged’’ by considering threshold levels for long-tailed nuclei (LTN), i.e., comets with the length over the 95th percentile of the distribution of the tail lengths for each sample after exposure to quercetin compared to un-exposed control sample. Results showed, that the percentage of LTN also increased after the quercetin treatment compared to un-exposed control. Based on the gained results, present study has provided evidence supporting the potential of using quercetin in anticancer therapy against bladder cancer.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA