Naslov
Anti-cancer effect of two novel palladium (II) complexes on human leukemia cell lines

Autori
Križnik, Bojana

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Zagreb, Hrvatska

Datum
21.10

Godina
2013

Stranica
49

Mentor
Marijanović, Inga

Neposredni voditelj
Antunović, Maja

Ključne riječi
leukemias; palladium (II) complexes; apoptosis; PARP; cytotoxicity

Sažetak
Effective treatments for human leukemias are in development, but so far none of them is completely satisfying. It was recently reported that palladium complexes have a significant anti-cancer activity as well as lower toxicity compared to some clinically used chemotherapeutics. Anti-cancer activities of two novel palladium (II) complexes [Pd(sac)(terpy)](sac)·4H2O and [PdCl(terpy)](sac)·2H2O were tested against three human leukemia cell lines: Jurkat, MOLT-4 and THP-1 and in comparison to cisplatin and adriamycin. Cytotoxic effect of drugs was determined with the MTT assay. Cell apoptosis was assessed with FITC-Annexin/PI staining for flow cytometry, and caspase-3 colorimetric assay. Furthermore, PARP cleavage was examined to elucidate whether treatments induce apoptotic or necrotic response. Both complexes exhibited a significant dose-dependent antigrowth effect in vitro. The complexes predominately induced apoptosis, but necrosis was also observed. In vitro results have shown that palladium (II) complexes may be a potential anti-cancer agents for treating human leukemias, but further analysis to determine putative mechanism of action and in vivo studies on animal models are essential.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA