Naslov
Trefoil family factor 3 expression in rat kidney 12 months after unilateral nephrectomy

Autori
Oršić Fric, Vlasta ; Mihalj, Martina ; Tolušić Levak, Maja ; Moguš, Mate ; Baus Lončar, Mirela ; Drenjančević, Ines ; Belovari, Tatjana Zibar, Lada

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Periodicum biologorum, Vol 115, suppl 2 / Drenjančević, Ines - Zagreb : Hrvatsko prirodoslovno društvo ; Institut Ruđer Bošković ; Laser Plus, 2013, 44-44

Skup
3.Congress of Croatian Physiological Society and 1.Regional Congress of the Physiological Societies

Mjesto i datum
Rijeka, Hrvatska, 13.09.2013. - 15.09.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chronic kidney disease; unilateral nephrectomy; trefoil family factor 3

Sažetak
Trefoil factor family 3 (TFF3) has been suggested as a good urinary biomarker for early detection of kidney tubular injury. We measured TFF3 expression in an animal model of chronic kidney disease (CKD) caused by unilateral nephrectomy (UN). 36 female Sprague Dawley rats were randomized into the three groups: unilaterally nephrectomized (A), sham operated (B) and naive rats (C). 12 months after UN the rats were sacrificed and the kidneys were taken for immunohistochemical analysis. Kidney function was followed up by repeated measurements of serum creatinine, sodium and potassium concentrations. Impairment of kidney function and development of CKD was proven by significantly increased serum creatinine concentrations in UN rats when compared to both control groups (A=40.3±5.9 ; B=29.1±4.4 ; C=29.6±2.9 ; p≤0.001. ANOVA). In the remaining kidneys of UN rats, the HE and PAS stained sections analysis revealed kidney tissue damage, including the loss of functional tissue, fibrosis, and lymphocyte infiltration. TFF3 was detected in all parts of the kidney tubular segment. However, its expression did not differ between remaining kidneys in UN rats and both control groups, nor to the young kidneys removed during the nephrectomy. Thus, our results suggest that the kidney expression of TFF3 is a weak biomarker of CKD.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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