Naslov
Characterization of Selected Gram-negative Foodborne Pathogens in Foods of Animal Origin Illegally Imported to the European Union

Autori
Nagy, Bela ; Smole-Mozina, Sonja ; Kovac, Jasna ; Wagner, Martin ; Schroder, Dagmar, Strauss, Anja ; Schlager, Sabine ; Beutlich, Janine ; Appel, Bernd ; Prukner-Radovcic, Estella ; Lusicky, Marija ; Cimerman, Mojca ; Toth, Istvan ; Kugler, Renata, Szmolka, Ama

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts - European Symposium on Food Safety 2014 / - Budimpešta : Intgernational Association for Food Protection, 2014

Skup
2014 European Symposium on Food Safety

Mjesto i datum
Budimpešta, Mađarska, 07.05.2014. - 09.05.2014

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Gram-negative Foodborne Pathogens; Foods of Animal Origin; Illegally Imported

Sažetak
Introduction. Uncharacterized types of pathogens with genetic traits exotic to Europe could be transferred from third countries into the EU with illegal food import. Purpose. The WP1 group of EU FP 7th Project (2012-2014) “Protection of consumers by microbial risk mitigation.” (PROMISE) aims to highlight some general characteristics of exogenous foodborne Gram-negative microbes of animal origin and estimate their unknown risks for human. Methods. Foods of animal origin confiscated at airports and groundports from 5 of the 10 participating countries: Austria, Croatia, Germany, Hungary and Slovenia were tested for the presence of foodborne pathogens by using standardized guidelines (ISO: for Campylobacter and for O157 VTEC, and agreed protocols for non-O157 VTEC). Results of phenotypic and genotypic characteristics of selected Gram negative foodborne pathogens (Campylobacter, verotoxic-, and multiresistant E. coli) from >1800 confiscated food samples are presented. Concerning Campylobacter, more than 180 suspected isolates from Partners were studied, but only two strains of Campylobacter jejuni isolated by Slovenian Partner were finally confirmed. Both strains represented raw chicken meat and were multidrug resistant. They were characterized by MLST, flaA, PFGE and binary PCR typing and were included in further epidemiological studies of antimicrobial resistance spreading in C. jejuni. O157 VTEC was not detected, but all together 17 strains of non-O157 VTEC from 3 Partners were identified, mainly from food of ruminant origin. Phenotypic and genotypic (microarray) analysis of VTEC indicated low rate of antimicrobial resistance but an abundance of E. coli fitness and virulence genes including stx and eae. In contrast, the 29 strains of multiresistant E. coli were characterized by a low numbers of virulence genes but by a higher number of non-exotic antimicrobial resistance genes and by class 1 integrons. Significance. It seems, that the above results should not raise major food safety concerns. Acknowledgment is due to PROMISE Project-no.265877.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA