A simple optical configuration for cell tracking by dark-field microscopy

Antolović, Vlatka; Marinović, Maja; Filić, Vedrana; Weber, Igor

doi:10.1016/j.mimet.2014.06.006

Pregled bibliografske jedinice broj: 704216

A simple optical configuration for cell tracking by dark-field microscopy

Antolović, Vlatka; Marinović, Maja; Filić, Vedrana; Weber, Igor

A simple optical configuration for cell tracking by dark-field microscopy // Journal of microbiological methods, 104 (2014), 9-11 doi:10.1016/j.mimet.2014.06.006 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 704216 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
A simple optical configuration for cell tracking by dark-field microscopy

Autori
Antolović, Vlatka ; Marinović, Maja ; Filić, Vedrana ; Weber, Igor

Izvornik
Journal of microbiological methods (0167-7012) 104 (2014); 9-11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
dark-field microscopy; annular aperture; long-working-distance condenser; cell migration; tracking; dictyostelium discoideum

Sažetak
We describe a simple optical configuration for dark-field microscopy at low magnification, realized with the use of standard microscope components. An inherent high contrast makes this method attractive for computer-assisted tracking and counting of microorganisms. We applied this setup for dark-field microscopy to measure the speed of migrating Dictyostelium amoebae.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA

Projekti:
098-0982913-2858 - Regulacija dinamike citoskeleta u kretanju i diobi stanica (Weber, Igor, MZOS ) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Vedrana Filić Mileta (autor)