Naslov
Multilocus sequence analysis of ‘Candidatus Phytoplasma asteris’ strain and the genome analysis of Turnip mosaic virus co-infecting oilseed rape

Autori
Šeruga Musić, Martina ; Huy Duc Nguyen ; Černi, Silvija ; Mamula, Đorđe ; Ohshima, Kazusato ; Škorić, Dijana

Izvornik
Journal of applied microbiology (1364-5072) 117 (2014), 3; 774-785

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
amp; groEL; rp gene; secY; tufB; TuMV genome

Sažetak
Molecular characterization of a pathogenic complex infecting winter oilseed rape (Brassica napus ssp. oleifera (DC.) Metzg.) plants showing typical rape phyllody symptoms along with some atypical changes. Phytoplasma (‘Candidatus Phytoplasma’) presence was confirmed by PCR-RFLP and 16S rRNA gene sequencing. Phylogenetic analyses of phytoplasma amp, tufB, secY, groEL and ribosomal protein genes confirmed its affiliation to the ‘Ca. P. asteris’ species. However, in the amp gene encoding a specific protein crucial for insect transmission specificity, significant SNPs were found. Biological and serological tests revealed the co-infection with Turnip mosaic virus (TuMV). The phylogenetic analysis of full TuMV genome sequence, the first reported from the Balkans, classified it into the world-B phylogenetic lineage. A pathogenic complex consisting of ‘Ca. P. asteris’ and TuMV found to co-infect oilseed rape plants for the first time was molecularly characterized. Significance and Impact of the Study: Rape phyllody is a serious problem in rapeseed production. The molecular information from this first multi-gene analysis of ‘Ca. P. asteris’ strain associated with rape phyllody as well as the first report of the complete sequence of TuMV isolate from the Balkans is a starting point for understanding the disease complexity and management.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA