Naslov
HIGHLY POROUS HYDROXYAPATITE/POLYCAPROLACTONE COMPOSITE SCAFFOLD: PHYSICOCHEMICAL AND IN VITRO BIOLOGICAL PROPERTIES

Autori
Milovac, Dajana ; Gamboa-Martínez, Tatiana C. ; Gallego Ferrer, Gloria ; Ivanković, Marica ; Ivanković, Hrvoje

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Skup
16th European Conference on Composite Materials, ECCM16

Mjesto i datum
Sevilla, Španjolska, 22.06.2014. - 26.06.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
poly(ε-caprolactone); hydroxyapatite; scaffold; tissue engineering

Sažetak
Hydrothermal transformation of aragonitic cuttlefish bone into hydroxyapatite (HAp) was performed at 200oC retaining the natural well interconnected porous structure. The scaffold was coated with a poly(ε-caprolactone) (PCL) using vacuum impregnation technique. The prepared scaffolds were characterized by Fourier transform infra-red spectroscopy (FTIR), X-ray diffraction analysis (XRD), and scanning electron microscopy (SEM). The mechanical properties of scaffolds were assessed under compression. Biological characterization of scaffolds was performed using the preosteoblastic cell line MC3T3-E1. The attachment and spreading of preosteoblasts on scaffolds were observed by Live/Dead kit staining. DNA content on HAp/PCL scaffold was significantly higher compared to porous HAp scaffolds. The osteoblastic differentiation of the cells was evaluated by determining ALP activity. The results demonstrate the potential of the HAp/PCL scaffold to be used in bone tissue engineering.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo, Temeljne tehničke znanosti

POVEZANOST RADA