Naslov
LOCATION AND mRNA EXPRESSION OF HYPOXIA-INDUCIBLE FACTOR-1 ALPHA IN KIDNEYS OF FEMALE SPRAGUE-DAWLEY RATS ONE YEAR AFTER UNILATERAL NEPHRECTOMY

Autori
Novak, Sanja ; Cosic, Anita ; Mihalj, Martina ; Jelakovic, Bojan ; Tolusic Levak, Maja ; Orsic Fric, Vlasta ; Mogus, Mate ; Zibar, Lada ; Drenjancevic, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of Hypertension, ESH-ISH Hypertension 2014, Abstract Book / - , 2014

Skup
Joint Meeting ESH-ISH Hypertension 2014

Mjesto i datum
Atena, Grčka, 13.06.2014. - 16.06.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
HIF-1α; nephrectomy

Sažetak
OBJECTIVE: Hypoxia inducible factor-1 alpha (HIF- 1α) is a transcription factor which regulates cell response to hypoxia, leading to transcription of many genes involved in energy metabolism, angiogenesis, and apoptosis. In patients undergoing unilateral or bilateral nephrectomy due to renal or urothelial cell carcinoma, or renal abscess, lower expression of HIF-1α is related to higher kidney fibrosis and lower estimated glomerular filtration of remnant kidney. After nephrectomy, remnant kidney enlarges for 25%, but if there is any role of HIF-1α in enlargement is unknown. Interestingly, HIF-1α is upregulated in hypertrophic cardiomyopathy. The objective of this study is to explore the HIF-1α mRNA expression and localization in remnant kidney of unilaterally nephrectomized, otherwise healthy female Sprague– Dawley rats. DESIGN AND METHODS: In this study 15 female Sprague-Dawley rats (4 months of age) were randomly divided into three groups: unilaterally nephrectomised, sham operated and naïve rats. One year after the nephrectomy, all rats were sacrificed and remained kidneys were weighted and further analyzed. HIF-1α mRNA expression was performed by real-time PCR ; mRNA expression of HIF-1α was normalized to HPRT housekeeping gene and analyzed by REST 2009. HIF-1α protein expression and distribution in kidneys was assessed by immunohistochemistry. RESULTS: There is a significant downregulation of HIF-1α gene in remnant kidneys of nephrectomized rats compared to naïve rats. There was no difference of HIF-1 mRNA expression in sham operated rats compared to other groups. In nephrectomised rats, immunohistochemistry showed that HIF-1α is expressed in proximal tubules, distal tubules and glomeruli, but highest expression was in proximal tubules. In medulla, HIF-1αis expressed in collecting tubules and with lower expression in loop of Henle of nephrectomised rat kidneys, compared to other parts of kidney. Destruction of parenchyma is present in kidney medulla of nephrectomised rats only. Hypertrophy of remnant kidney was present in nephrectomised rats with significantly higher kidney weight compared to sham operated and naïve rats. CONCLUSIONS: Our results show lower mRNA expression of HIF-1α which may be related to destruction of parenchyma present in medulla of unilaterally nephrectomized rats, but hypertrophy is not linked to HIF-1α expression.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA