Naslov
Lipid domains in the exoplasmic and cytoplasmic leaflet of human erythrocyte membrane ; a spin label approach

Autori
Žuvić-Butorac, Marta ; Mueller, Peter ; Pomorski, Tomas ; Libera, Janette ; Herrmann, Andreas ; Schara, Milan

Izvornik
European Biophysics Journal (0175-7571) 28 (1999); 302-311

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
erythrocyte; plasma membrane; leaflet; lipid domains; spin label
(erythrocyte; plasma membrane; leaflet; lipid domains; spin labelk)

Sažetak
The existence of different lipid domains in the monolayers of the human erythrocyte membrane was investigated at 4 C by employing spin-labelled phospholipid analogues. Spectra of analogues located exclusivelly either in the exoplasmic either in the cytoplasmic leaflet of erythrocyte membranes were recorded.Spectra were simulated by variation of order parameter describing the average amplitude of motion of the long molecular axis of the nitorgen 2pP orbital of the spin label and of respective corelation times. For both leaflets at least three components were required to fit experimental spectra, differing mainly in the order parameter. While the pšarameters of each component are not very different between both membrane halves, the relative contribution of each component to the spectrum is different between the exoplasmic and cytoplasmic leaflet. The order parameter of the most fluid component, preasumbly resembling the lipid bulk phase, is smaller in the cytoplasmic leaflet in comparison to the exoplasmic one. the alteral coexistance of different lipid domains in the human red blood cell membrane is concluded. The molecular nature of those domains is discussed.

Izvorni jezik
Engleski

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